Journal Article

PreJuSER-11684

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

Smith, A.-S. ; Fenz, S. F.FZJ* ; Sengupta, K.

2010
EDP Sciences Les Ulis

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Please use a persistent id in citations: doi:10.1209/0295-5075/89/28003

Abstract: Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented. Copyright (C) EPLA, 2010

Keyword(s): J

Note: We are grateful to S. GONNENWEIN for giving access to her data (figs. 1 and 4). We thank R. Merkel, E. SACKMANN, U. SEIFERT, C. MONZEL and L. LIMOZIN for stimulating discussions and R. Merkel for careful reading of the manuscript. A-SS was funded by DFG-SE 1119/2-1. KS and SFF thank R. MERKEL for support.

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Contributing Institute(s):

1. Biomechanik (IBN-4)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2010

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Database coverage:
JCR ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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