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@ARTICLE{Smith:11684,
author = {Smith, A.-S. and Fenz, S.F. and Sengupta, K.},
title = {{I}nferring spatial organization of bonds within adhesion
clusters by exploiting fluctuations of soft interfaces},
journal = {epl},
volume = {89},
issn = {0302-072X},
address = {Les Ulis},
publisher = {EDP Sciences},
reportid = {PreJuSER-11684},
pages = {28003},
year = {2010},
note = {We are grateful to S. GONNENWEIN for giving access to her
data (figs. 1 and 4). We thank R. Merkel, E. SACKMANN, U.
SEIFERT, C. MONZEL and L. LIMOZIN for stimulating
discussions and R. Merkel for careful reading of the
manuscript. A-SS was funded by DFG-SE 1119/2-1. KS and SFF
thank R. MERKEL for support.},
abstract = {Detecting the organization of bonds within adhesion domains
connecting two interacting membranes is, at present,
extremely challenging. Herein we present a technique, based
on Reflection Interference Contrast Microscopy, which uses
spontaneous thermal fluctuations of a soft interface as a
tool to identify the organization of specific
ligand-receptor bonds. The key is a time-resolved analysis
of micro-interferometric data that systematically quantifies
fluctuations and enables the detection of their suppression
due to the formation of bonds, which, in turn, allows the
identification of the bond organization without the use of
fluorescent labelling. The identification of a new type of
bond organization characterized by sparsely distributed
bonds, as well as detection of pinning centres of nanometric
size is presented. Copyright (C) EPLA, 2010},
keywords = {J (WoSType)},
cin = {IBN-4},
ddc = {530},
cid = {I:(DE-Juel1)VDB802},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK505},
shelfmark = {Physics, Multidisciplinary},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000274848500034},
doi = {10.1209/0295-5075/89/28003},
url = {https://juser.fz-juelich.de/record/11684},
}