Journal Article

PreJuSER-12054

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Diffusion in single supported lipid bilayers studied by quasi-elastic neutron scattering

Armstrong, C. L. ; Kaye, M. D. ; Zamponi, M.FZJ* ; Mamontov, E. ; Tyagi, M. ; Jenkins, T. ; Rheinstädter, M. C.

2010
Royal Society of Chemistry (RSC) Cambridge

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Please use a persistent id in citations: doi:10.1039/c0sm00637h

Abstract: It seems to be increasingly accepted that the diversity and composition of lipids play an important role in the function of biological membranes. A prime example of this is the case of lipid rafts; regions enriched with certain types of lipids which are speculated to be relevant to the proper functioning of membrane embedded proteins. Although the dynamics of membrane systems have been studied for decades, the microscopic dynamics of lipid molecules, even in simple model systems, is still an active topic of debate. Neutron scattering has proven to be an important tool for accessing the relevant nanometre length scale and nano to picosecond time scales, thus providing complimentary information to macroscopic techniques. Despite their potential relevance for the development of functionalized surfaces and biosensors, the study of single supported membranes using neutron scattering poses the challenge of obtaining relevant dynamic information from a sample with minimal material. Using state of the art neutron instrumentation we were, for the first time, able to model lipid diffusion in single supported lipid bilayers. We find that the diffusion coefficient for the single bilayer system is comparable to the multi-lamellar lipid system. More importantly, the molecular mechanism for lipid motion in the single bilayer was found to be a continuous diffusion, rather than the flow-like ballistic motion reported in the stacked membrane system. We observed an enhanced diffusion at the nearest neighbour distance of the lipid molecules. The enhancement and change of character of the diffusion can most likely be attributed to the effect the supporting substrate has on the lipid organization.

Keyword(s): J

Note: This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC), the National Research Council Canada (NRC) and the Canada Foundation for Innovation (CFI). This work utilized facilities at the NIST Center for Neutron Research supported in part by the NSF under agreement No. DMR-0454672. Research at Oak Ridge National Laboratory's Spallation Neutron Source was sponsored by the Scientific User Facilities Division, Office of Basic Energy Sciences, U. S. Department of Energy.

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Contributing Institute(s):

1. Streumethoden (IFF-4)
2. Neutronenstreuung (IFF-5)
3. JCNS (Jülich Centre for Neutron Science JCNS (JCNS) ; JCNS)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
2. Großgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI) (P55)

Appears in the scientific report 2010

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