%0 Journal Article
%A Blombach, B.
%A Schreiner, M. E.
%A Bartek, T.
%A Oldiges, M.
%A Eikmanns, B. J.
%T Corynebacterium glutamicum tailored for high-yield L-valine production
%J Applied Microbiology and Biotechnology
%V 79
%@ 0175-7598
%C Berlin
%I Springer
%M PreJuSER-1232
%P 471 - 479
%D 2008
%Z Record converted from VDB: 12.11.2012
%X We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.
%K Bacterial Proteins: genetics
%K Bacterial Proteins: metabolism
%K Biomass
%K Biosynthetic Pathways
%K Corynebacterium glutamicum: enzymology
%K Corynebacterium glutamicum: genetics
%K Corynebacterium glutamicum: metabolism
%K Fermentation
%K Gene Expression
%K Genetic Engineering
%K Ketol-Acid Reductoisomerase: genetics
%K Ketol-Acid Reductoisomerase: metabolism
%K Oxidoreductases: genetics
%K Oxidoreductases: metabolism
%K Pyruvate Dehydrogenase Complex: genetics
%K Pyruvate Dehydrogenase Complex: metabolism
%K Transaminases: genetics
%K Transaminases: metabolism
%K Valine: metabolism
%K Bacterial Proteins (NLM Chemicals)
%K Pyruvate Dehydrogenase Complex (NLM Chemicals)
%K Valine (NLM Chemicals)
%K Oxidoreductases (NLM Chemicals)
%K Ketol-Acid Reductoisomerase (NLM Chemicals)
%K Transaminases (NLM Chemicals)
%K branched-chain-amino-acid transaminase (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:18379776
%U <Go to ISI:>//WOS:000256026800016
%R 10.1007/s00253-008-1444-z
%U https://juser.fz-juelich.de/record/1232