Journal Article

PreJuSER-1232

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Corynebacterium glutamicum tailored for high-yield L-valine production

Blombach, B. ; Schreiner, M. E. ; Bartek, T.FZJ* ; Oldiges, M.FZJ* ; Eikmanns, B. J.

2008
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00253-008-1444-z

Abstract: We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.

Keyword(s): Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Biomass (MeSH) ; Biosynthetic Pathways (MeSH) ; Corynebacterium glutamicum: enzymology (MeSH) ; Corynebacterium glutamicum: genetics (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; Fermentation (MeSH) ; Gene Expression (MeSH) ; Genetic Engineering (MeSH) ; Ketol-Acid Reductoisomerase: genetics (MeSH) ; Ketol-Acid Reductoisomerase: metabolism (MeSH) ; Oxidoreductases: genetics (MeSH) ; Oxidoreductases: metabolism (MeSH) ; Pyruvate Dehydrogenase Complex: genetics (MeSH) ; Pyruvate Dehydrogenase Complex: metabolism (MeSH) ; Transaminases: genetics (MeSH) ; Transaminases: metabolism (MeSH) ; Valine: metabolism (MeSH) ; Bacterial Proteins ; Pyruvate Dehydrogenase Complex ; Valine ; Oxidoreductases ; Ketol-Acid Reductoisomerase ; Transaminases ; branched-chain-amino-acid transaminase ; J ; Corynebacterium glutamicum (auto) ; L-valine production (auto) ; pyruvate dehydrogenase complex (auto) ; pyruvate : quinone oxidoreductase (auto) ; phosphoglucose isomerase (auto) ; pyruvate carboxylase (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biotechnologie 2 (IBT-2)

Research Program(s):

1. Biotechnologie (PBT)

Appears in the scientific report 2008

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Database coverage:
; JCR ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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