000001232 001__ 1232
000001232 005__ 20190625111452.0
000001232 0247_ $$2pmid$$apmid:18379776
000001232 0247_ $$2DOI$$a10.1007/s00253-008-1444-z
000001232 0247_ $$2WOS$$aWOS:000256026800016
000001232 0247_ $$2ISSN$$a0175-7598
000001232 0247_ $$2altmetric$$aaltmetric:21800565
000001232 037__ $$aPreJuSER-1232
000001232 041__ $$aeng
000001232 082__ $$a570
000001232 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000001232 1001_ $$0P:(DE-HGF)0$$aBlombach, B.$$b0
000001232 245__ $$aCorynebacterium glutamicum tailored for high-yield L-valine production
000001232 260__ $$aBerlin$$bSpringer$$c2008
000001232 300__ $$a471 - 479
000001232 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000001232 3367_ $$2DataCite$$aOutput Types/Journal article
000001232 3367_ $$00$$2EndNote$$aJournal Article
000001232 3367_ $$2BibTeX$$aARTICLE
000001232 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000001232 3367_ $$2DRIVER$$aarticle
000001232 440_0 $$0555$$aApplied Microbiology and Biotechnology$$v79$$x0175-7598$$y3
000001232 500__ $$aRecord converted from VDB: 12.11.2012
000001232 520__ $$aWe recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.
000001232 536__ $$0G:(DE-Juel1)FUEK410$$2G:(DE-HGF)$$aBiotechnologie$$cPBT$$x0
000001232 588__ $$aDataset connected to Web of Science, Pubmed
000001232 650_2 $$2MeSH$$aBacterial Proteins: genetics
000001232 650_2 $$2MeSH$$aBacterial Proteins: metabolism
000001232 650_2 $$2MeSH$$aBiomass
000001232 650_2 $$2MeSH$$aBiosynthetic Pathways
000001232 650_2 $$2MeSH$$aCorynebacterium glutamicum: enzymology
000001232 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000001232 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000001232 650_2 $$2MeSH$$aFermentation
000001232 650_2 $$2MeSH$$aGene Expression
000001232 650_2 $$2MeSH$$aGenetic Engineering
000001232 650_2 $$2MeSH$$aKetol-Acid Reductoisomerase: genetics
000001232 650_2 $$2MeSH$$aKetol-Acid Reductoisomerase: metabolism
000001232 650_2 $$2MeSH$$aOxidoreductases: genetics
000001232 650_2 $$2MeSH$$aOxidoreductases: metabolism
000001232 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: genetics
000001232 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: metabolism
000001232 650_2 $$2MeSH$$aTransaminases: genetics
000001232 650_2 $$2MeSH$$aTransaminases: metabolism
000001232 650_2 $$2MeSH$$aValine: metabolism
000001232 650_7 $$00$$2NLM Chemicals$$aBacterial Proteins
000001232 650_7 $$00$$2NLM Chemicals$$aPyruvate Dehydrogenase Complex
000001232 650_7 $$07004-03-7$$2NLM Chemicals$$aValine
000001232 650_7 $$0EC 1.-$$2NLM Chemicals$$aOxidoreductases
000001232 650_7 $$0EC 1.1.1.86$$2NLM Chemicals$$aKetol-Acid Reductoisomerase
000001232 650_7 $$0EC 2.6.1.-$$2NLM Chemicals$$aTransaminases
000001232 650_7 $$0EC 2.6.1.42$$2NLM Chemicals$$abranched-chain-amino-acid transaminase
000001232 650_7 $$2WoSType$$aJ
000001232 65320 $$2Author$$aCorynebacterium glutamicum
000001232 65320 $$2Author$$aL-valine production
000001232 65320 $$2Author$$apyruvate dehydrogenase complex
000001232 65320 $$2Author$$apyruvate : quinone oxidoreductase
000001232 65320 $$2Author$$aphosphoglucose isomerase
000001232 65320 $$2Author$$apyruvate carboxylase
000001232 7001_ $$0P:(DE-HGF)0$$aSchreiner, M. E.$$b1
000001232 7001_ $$0P:(DE-Juel1)VDB59554$$aBartek, T.$$b2$$uFZJ
000001232 7001_ $$0P:(DE-Juel1)129053$$aOldiges, M.$$b3$$uFZJ
000001232 7001_ $$0P:(DE-HGF)0$$aEikmanns, B. J.$$b4
000001232 773__ $$0PERI:(DE-600)1464336-4$$a10.1007/s00253-008-1444-z$$gVol. 79, p. 471 - 479$$p471 - 479$$q79<471 - 479$$tApplied Microbiology and Biotechnology$$v79$$x0175-7598$$y2008
000001232 8567_ $$uhttp://dx.doi.org/10.1007/s00253-008-1444-z
000001232 909CO $$ooai:juser.fz-juelich.de:1232$$pVDB
000001232 9131_ $$0G:(DE-Juel1)FUEK410$$baußerhalb PoF$$kPBT$$lohne FE$$vBiotechnologie$$x0
000001232 9141_ $$y2008
000001232 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000001232 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000001232 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000001232 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000001232 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000001232 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000001232 9201_ $$0I:(DE-Juel1)VDB56$$gIBT$$kIBT-2$$lBiotechnologie 2$$x0$$zab 31.10.10 weitergeführt IBG-1
000001232 970__ $$aVDB:(DE-Juel1)102182
000001232 980__ $$aVDB
000001232 980__ $$aConvertedRecord
000001232 980__ $$ajournal
000001232 980__ $$aI:(DE-Juel1)IBG-1-20101118
000001232 980__ $$aUNRESTRICTED
000001232 981__ $$aI:(DE-Juel1)IBG-1-20101118