TY  - JOUR
AU  - Blombach, B.
AU  - Schreiner, M. E.
AU  - Bartek, T.
AU  - Oldiges, M.
AU  - Eikmanns, B. J.
TI  - Corynebacterium glutamicum tailored for high-yield L-valine production
JO  - Applied Microbiology and Biotechnology
VL  - 79
SN  - 0175-7598
CY  - Berlin
PB  - Springer
M1  - PreJuSER-1232
SP  - 471 - 479
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested.
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: metabolism
KW  - Biomass
KW  - Biosynthetic Pathways
KW  - Corynebacterium glutamicum: enzymology
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: metabolism
KW  - Fermentation
KW  - Gene Expression
KW  - Genetic Engineering
KW  - Ketol-Acid Reductoisomerase: genetics
KW  - Ketol-Acid Reductoisomerase: metabolism
KW  - Oxidoreductases: genetics
KW  - Oxidoreductases: metabolism
KW  - Pyruvate Dehydrogenase Complex: genetics
KW  - Pyruvate Dehydrogenase Complex: metabolism
KW  - Transaminases: genetics
KW  - Transaminases: metabolism
KW  - Valine: metabolism
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Pyruvate Dehydrogenase Complex (NLM Chemicals)
KW  - Valine (NLM Chemicals)
KW  - Oxidoreductases (NLM Chemicals)
KW  - Ketol-Acid Reductoisomerase (NLM Chemicals)
KW  - Transaminases (NLM Chemicals)
KW  - branched-chain-amino-acid transaminase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18379776
UR  - <Go to ISI:>//WOS:000256026800016
DO  - DOI:10.1007/s00253-008-1444-z
UR  - https://juser.fz-juelich.de/record/1232
ER  -