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@ARTICLE{Blombach:1232,
      author       = {Blombach, B. and Schreiner, M. E. and Bartek, T. and
                      Oldiges, M. and Eikmanns, B. J.},
      title        = {{C}orynebacterium glutamicum tailored for high-yield
                      {L}-valine production},
      journal      = {Applied Microbiology and Biotechnology},
      volume       = {79},
      issn         = {0175-7598},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {PreJuSER-1232},
      pages        = {471 - 479},
      year         = {2008},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {We recently engineered the wild type of Corynebacterium
                      glutamicum for the growth-decoupled production of L: -valine
                      from glucose by inactivation of the pyruvate dehydrogenase
                      complex and additional overexpression of the ilvBNCE genes,
                      encoding the L-valine biosynthetic enzymes acetohydroxyacid
                      synthase, isomeroreductase, and transaminase B. Based on the
                      first generation of pyruvate-dehydrogenase-complex-deficient
                      C. glutamicum strains, a second generation of high-yield
                      L-valine producers was constructed by successive deletion of
                      the genes encoding pyruvate:quinone oxidoreductase,
                      phosphoglucose isomerase, and pyruvate carboxylase and
                      overexpression of ilvBNCE. In fed-batch fermentations at
                      high cell densities, the newly constructed strains produced
                      up to 410 mM (48 g/l) L-valine, showed a maximum yield of
                      0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the
                      production phase and, in contrast to the first generation
                      strains, excreted neither pyruvate nor any other by-product
                      tested.},
      keywords     = {Bacterial Proteins: genetics / Bacterial Proteins:
                      metabolism / Biomass / Biosynthetic Pathways /
                      Corynebacterium glutamicum: enzymology / Corynebacterium
                      glutamicum: genetics / Corynebacterium glutamicum:
                      metabolism / Fermentation / Gene Expression / Genetic
                      Engineering / Ketol-Acid Reductoisomerase: genetics /
                      Ketol-Acid Reductoisomerase: metabolism / Oxidoreductases:
                      genetics / Oxidoreductases: metabolism / Pyruvate
                      Dehydrogenase Complex: genetics / Pyruvate Dehydrogenase
                      Complex: metabolism / Transaminases: genetics /
                      Transaminases: metabolism / Valine: metabolism / Bacterial
                      Proteins (NLM Chemicals) / Pyruvate Dehydrogenase Complex
                      (NLM Chemicals) / Valine (NLM Chemicals) / Oxidoreductases
                      (NLM Chemicals) / Ketol-Acid Reductoisomerase (NLM
                      Chemicals) / Transaminases (NLM Chemicals) /
                      branched-chain-amino-acid transaminase (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:18379776},
      UT           = {WOS:000256026800016},
      doi          = {10.1007/s00253-008-1444-z},
      url          = {https://juser.fz-juelich.de/record/1232},
}