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| 001 | 1232 | ||
| 005 | 20190625111452.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:18379776 |
| 024 | 7 | _ | |2 DOI |a 10.1007/s00253-008-1444-z |
| 024 | 7 | _ | |2 WOS |a WOS:000256026800016 |
| 024 | 7 | _ | |2 ISSN |a 0175-7598 |
| 024 | 7 | _ | |a altmetric:21800565 |2 altmetric |
| 037 | _ | _ | |a PreJuSER-1232 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biotechnology & Applied Microbiology |
| 100 | 1 | _ | |a Blombach, B. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a Corynebacterium glutamicum tailored for high-yield L-valine production |
| 260 | _ | _ | |c 2008 |a Berlin |b Springer |
| 300 | _ | _ | |a 471 - 479 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Applied Microbiology and Biotechnology |x 0175-7598 |0 555 |y 3 |v 79 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested. |
| 536 | _ | _ | |a Biotechnologie |c PBT |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK410 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Bacterial Proteins: genetics |
| 650 | _ | 2 | |2 MeSH |a Bacterial Proteins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Biomass |
| 650 | _ | 2 | |2 MeSH |a Biosynthetic Pathways |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: enzymology |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: genetics |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: metabolism |
| 650 | _ | 2 | |2 MeSH |a Fermentation |
| 650 | _ | 2 | |2 MeSH |a Gene Expression |
| 650 | _ | 2 | |2 MeSH |a Genetic Engineering |
| 650 | _ | 2 | |2 MeSH |a Ketol-Acid Reductoisomerase: genetics |
| 650 | _ | 2 | |2 MeSH |a Ketol-Acid Reductoisomerase: metabolism |
| 650 | _ | 2 | |2 MeSH |a Oxidoreductases: genetics |
| 650 | _ | 2 | |2 MeSH |a Oxidoreductases: metabolism |
| 650 | _ | 2 | |2 MeSH |a Pyruvate Dehydrogenase Complex: genetics |
| 650 | _ | 2 | |2 MeSH |a Pyruvate Dehydrogenase Complex: metabolism |
| 650 | _ | 2 | |2 MeSH |a Transaminases: genetics |
| 650 | _ | 2 | |2 MeSH |a Transaminases: metabolism |
| 650 | _ | 2 | |2 MeSH |a Valine: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Bacterial Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Pyruvate Dehydrogenase Complex |
| 650 | _ | 7 | |0 7004-03-7 |2 NLM Chemicals |a Valine |
| 650 | _ | 7 | |0 EC 1.- |2 NLM Chemicals |a Oxidoreductases |
| 650 | _ | 7 | |0 EC 1.1.1.86 |2 NLM Chemicals |a Ketol-Acid Reductoisomerase |
| 650 | _ | 7 | |0 EC 2.6.1.- |2 NLM Chemicals |a Transaminases |
| 650 | _ | 7 | |0 EC 2.6.1.42 |2 NLM Chemicals |a branched-chain-amino-acid transaminase |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a Corynebacterium glutamicum |
| 653 | 2 | 0 | |2 Author |a L-valine production |
| 653 | 2 | 0 | |2 Author |a pyruvate dehydrogenase complex |
| 653 | 2 | 0 | |2 Author |a pyruvate : quinone oxidoreductase |
| 653 | 2 | 0 | |2 Author |a phosphoglucose isomerase |
| 653 | 2 | 0 | |2 Author |a pyruvate carboxylase |
| 700 | 1 | _ | |a Schreiner, M. E. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Bartek, T. |b 2 |u FZJ |0 P:(DE-Juel1)VDB59554 |
| 700 | 1 | _ | |a Oldiges, M. |b 3 |u FZJ |0 P:(DE-Juel1)129053 |
| 700 | 1 | _ | |a Eikmanns, B. J. |b 4 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |0 PERI:(DE-600)1464336-4 |a 10.1007/s00253-008-1444-z |g Vol. 79, p. 471 - 479 |p 471 - 479 |q 79<471 - 479 |t Applied Microbiology and Biotechnology |v 79 |x 0175-7598 |y 2008 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1007/s00253-008-1444-z |
| 909 | C | O | |o oai:juser.fz-juelich.de:1232 |p VDB |
| 913 | 1 | _ | |k PBT |v Biotechnologie |l ohne FE |b außerhalb PoF |0 G:(DE-Juel1)FUEK410 |x 0 |
| 914 | 1 | _ | |y 2008 |
| 915 | _ | _ | |a JCR/ISI refereed |0 StatID:(DE-HGF)0010 |2 StatID |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
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| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 920 | 1 | _ | |k IBT-2 |l Biotechnologie 2 |g IBT |z ab 31.10.10 weitergeführt IBG-1 |0 I:(DE-Juel1)VDB56 |x 0 |
| 970 | _ | _ | |a VDB:(DE-Juel1)102182 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
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