000013093 001__ 13093
000013093 005__ 20200402205936.0
000013093 0247_ $$2pmid$$apmid:20364135
000013093 0247_ $$2DOI$$a10.1038/nnano.2010.44
000013093 0247_ $$2WOS$$aWOS:000278264300015
000013093 0247_ $$2altmetric$$aaltmetric:3311239
000013093 037__ $$aPreJuSER-13093
000013093 041__ $$aeng
000013093 082__ $$a600
000013093 084__ $$2WoS$$aNanoscience & Nanotechnology
000013093 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000013093 1001_ $$0P:(DE-HGF)0$$aKagan, V.E.$$b0
000013093 245__ $$aCarbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation
000013093 260__ $$aLondon [u.a.]$$bNature Publishing Group$$c2010
000013093 300__ $$a354 - 359
000013093 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000013093 3367_ $$2DataCite$$aOutput Types/Journal article
000013093 3367_ $$00$$2EndNote$$aJournal Article
000013093 3367_ $$2BibTeX$$aARTICLE
000013093 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000013093 3367_ $$2DRIVER$$aarticle
000013093 440_0 $$023424$$aNature Nanotechnology$$v5$$x1748-3387$$y5
000013093 500__ $$aThis work was supported by grants from National Institute for Occupational Safety and Health (NIOSH) 011008282, National Institutes of Health HL70755, HL094488, U19AI068021, National Library of Medicine LM007994-05, National Occupational Research Agenda (NORA) 927000Y, 927Z1LU, Nanotechnology Research Center (NTRC) 927ZJHF, National Science Foundation (NSF) CAREER 0449117, Air Force Office of Scientific Research (AFOSR) FA9550-09-1-0478, 7th Framework Program of the European Commission (EC-FP7-NANOMMUNE-214281) and by the Science Foundation of Ireland, Strategic Research Cluster (SRC) BioNanointeract and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Higher Education Authority (HEA) and Programme for Research in Third-Level Institutions (PRTLI). The authors would like to thank Marcel Bruchez for assistance with dynamic light scattering experiments.
000013093 520__ $$aWe have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. However, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. Here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotubes in vitro, in neutrophils and to a lesser degree in macrophages. Molecular modelling suggests that interactions of basic amino acids of the enzyme with the carboxyls on the carbon nanotubes position the nanotubes near the catalytic site. Importantly, the biodegraded nanotubes do not generate an inflammatory response when aspirated into the lungs of mice. Our findings suggest that the extent to which carbon nanotubes are biodegraded may be a major determinant of the scale and severity of the associated inflammatory responses in exposed individuals.
000013093 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000013093 536__ $$0G:(EU-Grant)214281$$aNANOMMUNE - Comprehensive assessment of hazardous effects of engineered nanomaterials on the immune system (214281)$$c214281$$fFP7-NMP-2007-SMALL-1$$x1
000013093 588__ $$aDataset connected to Web of Science, Pubmed
000013093 650_2 $$2MeSH$$aAnimals
000013093 650_2 $$2MeSH$$aHumans
000013093 650_2 $$2MeSH$$aImmunoglobulin G: immunology
000013093 650_2 $$2MeSH$$aMice
000013093 650_2 $$2MeSH$$aMice, Inbred C57BL
000013093 650_2 $$2MeSH$$aModels, Molecular
000013093 650_2 $$2MeSH$$aNanotubes, Carbon: chemistry
000013093 650_2 $$2MeSH$$aNanotubes, Carbon: toxicity
000013093 650_2 $$2MeSH$$aNanotubes, Carbon: ultrastructure
000013093 650_2 $$2MeSH$$aNeutrophils: drug effects
000013093 650_2 $$2MeSH$$aNeutrophils: enzymology
000013093 650_2 $$2MeSH$$aPeroxidase: metabolism
000013093 650_2 $$2MeSH$$aPneumonia: chemically induced
000013093 650_2 $$2MeSH$$aPneumonia: pathology
000013093 650_2 $$2MeSH$$aReactive Oxygen Species: metabolism
000013093 650_2 $$2MeSH$$aSpectrophotometry, Infrared
000013093 650_2 $$2MeSH$$aSpectrum Analysis, Raman
000013093 650_7 $$00$$2NLM Chemicals$$aImmunoglobulin G
000013093 650_7 $$00$$2NLM Chemicals$$aNanotubes, Carbon
000013093 650_7 $$00$$2NLM Chemicals$$aReactive Oxygen Species
000013093 650_7 $$0EC 1.11.1.7$$2NLM Chemicals$$aPeroxidase
000013093 650_7 $$2WoSType$$aJ
000013093 7001_ $$0P:(DE-HGF)0$$aKonduru, N.V.$$b1
000013093 7001_ $$0P:(DE-HGF)0$$aFeng, W.$$b2
000013093 7001_ $$0P:(DE-HGF)0$$aAllen, B.L.$$b3
000013093 7001_ $$0P:(DE-HGF)0$$aConroy, J.$$b4
000013093 7001_ $$0P:(DE-HGF)0$$aVolkov, Y.$$b5
000013093 7001_ $$0P:(DE-HGF)0$$aVlasova, I.I.$$b6
000013093 7001_ $$0P:(DE-HGF)0$$aBelikova, N.A.$$b7
000013093 7001_ $$0P:(DE-HGF)0$$aYanamala, N.$$b8
000013093 7001_ $$0P:(DE-HGF)0$$aApralov, A$$b9
000013093 7001_ $$0P:(DE-HGF)0$$aTyurina, Y.Y.$$b10
000013093 7001_ $$0P:(DE-HGF)0$$aKisin, E.R.$$b11
000013093 7001_ $$0P:(DE-HGF)0$$aMurray, A.R.$$b12
000013093 7001_ $$0P:(DE-HGF)0$$aFranks, J.$$b13
000013093 7001_ $$0P:(DE-HGF)0$$aStolz, D.$$b14
000013093 7001_ $$0P:(DE-HGF)0$$aGou, P.$$b15
000013093 7001_ $$0P:(DE-HGF)0$$aShi, J.$$b16
000013093 7001_ $$0P:(DE-Juel1)VDB44599$$aKlein-Seetharaman, J.$$b17$$uFZJ
000013093 7001_ $$0P:(DE-HGF)0$$aFadeel, B.$$b18
000013093 7001_ $$0P:(DE-HGF)0$$aStar, A.$$b19
000013093 7001_ $$0P:(DE-HGF)0$$aShvedova, A.$$b20
000013093 773__ $$0PERI:(DE-600)2254964-X$$a10.1038/nnano.2010.44$$gVol. 5, p. 354 - 359$$p354 - 359$$q5<354 - 359$$tNature nanotechnology$$v5$$x1748-3387$$y2010
000013093 8567_ $$uhttp://dx.doi.org/10.1038/nnano.2010.44
000013093 909CO $$ooai:juser.fz-juelich.de:13093$$pec_fundedresources$$pVDB$$popenaire
000013093 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000013093 9141_ $$y2010
000013093 9131_ $$0G:(DE-Juel1)FUEK505$$aDE-HGF$$bSchlüsseltechnologien$$kP45$$lBiologische Informationsverarbeitung$$vBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$x0
000013093 9201_ $$0I:(DE-Juel1)ISB-2-20090406$$d31.12.2010$$gISB$$kISB-2$$lMolekulare Biophysik$$x0
000013093 970__ $$aVDB:(DE-Juel1)124920
000013093 980__ $$aVDB
000013093 980__ $$aConvertedRecord
000013093 980__ $$ajournal
000013093 980__ $$aI:(DE-Juel1)ICS-6-20110106
000013093 980__ $$aUNRESTRICTED
000013093 981__ $$aI:(DE-Juel1)IBI-7-20200312
000013093 981__ $$aI:(DE-Juel1)ICS-6-20110106
000013093 981__ $$aI:(DE-Juel1)ISB-2-20090406