Journal Article

PreJuSER-13093

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation

Kagan, V. E. ; Konduru, N. V. ; Feng, W. ; Allen, B. L. ; Conroy, J. ; Volkov, Y. ; Vlasova, I. I. ; Belikova, N. A. ; Yanamala, N. ; Apralov, A. ; Tyurina, Y. Y. ; Kisin, E. R. ; Murray, A. R. ; Franks, J. ; Stolz, D. ; Gou, P. ; Shi, J. ; Klein-Seetharaman, J.FZJ* ; Fadeel, B. ; Star, A. ; Shvedova, A.

2010
Nature Publishing Group London [u.a.]

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Please use a persistent id in citations: doi:10.1038/nnano.2010.44

Abstract: We have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. However, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. Here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotubes in vitro, in neutrophils and to a lesser degree in macrophages. Molecular modelling suggests that interactions of basic amino acids of the enzyme with the carboxyls on the carbon nanotubes position the nanotubes near the catalytic site. Importantly, the biodegraded nanotubes do not generate an inflammatory response when aspirated into the lungs of mice. Our findings suggest that the extent to which carbon nanotubes are biodegraded may be a major determinant of the scale and severity of the associated inflammatory responses in exposed individuals.

Keyword(s): Animals (MeSH) ; Humans (MeSH) ; Immunoglobulin G: immunology (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Models, Molecular (MeSH) ; Nanotubes, Carbon: chemistry (MeSH) ; Nanotubes, Carbon: toxicity (MeSH) ; Nanotubes, Carbon: ultrastructure (MeSH) ; Neutrophils: drug effects (MeSH) ; Neutrophils: enzymology (MeSH) ; Peroxidase: metabolism (MeSH) ; Pneumonia: chemically induced (MeSH) ; Pneumonia: pathology (MeSH) ; Reactive Oxygen Species: metabolism (MeSH) ; Spectrophotometry, Infrared (MeSH) ; Spectrum Analysis, Raman (MeSH) ; Immunoglobulin G ; Nanotubes, Carbon ; Reactive Oxygen Species ; Peroxidase ; J

Note: This work was supported by grants from National Institute for Occupational Safety and Health (NIOSH) 011008282, National Institutes of Health HL70755, HL094488, U19AI068021, National Library of Medicine LM007994-05, National Occupational Research Agenda (NORA) 927000Y, 927Z1LU, Nanotechnology Research Center (NTRC) 927ZJHF, National Science Foundation (NSF) CAREER 0449117, Air Force Office of Scientific Research (AFOSR) FA9550-09-1-0478, 7th Framework Program of the European Commission (EC-FP7-NANOMMUNE-214281) and by the Science Foundation of Ireland, Strategic Research Cluster (SRC) BioNanointeract and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Higher Education Authority (HEA) and Programme for Research in Third-Level Institutions (PRTLI). The authors would like to thank Marcel Bruchez for assistance with dynamic light scattering experiments.

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Contributing Institute(s):

1. Molekulare Biophysik (ISB-2)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
2. NANOMMUNE - Comprehensive assessment of hazardous effects of engineered nanomaterials on the immune system (214281) (214281)

Appears in the scientific report 2010

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