TY  - JOUR
AU  - Schwaab, Daniel
AU  - Zentis, Peter
AU  - Winter, Silke
AU  - Meffert, Simone
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
TI  - Generation of Protein Nanogradients by Microcontact Printing4
JO  - Japanese journal of applied physics
VL  - 52
SN  - 1347-4065
CY  - Tokyo
PB  - Inst. of Pure and Applied Physics
M1  - FZJ-2013-04574
SP  - 05DA19 
PY  - 2013
AB  - High resolution lithography combined with microcontact printing (µCP) by means of polyolefine polymer (POP) stamps enabled to create protein gradient patterns. By this means, discrete purely biochemical gradients of extracellular matrix proteins were fabricated. It was possible to adjust independently both the size of elements of a protein pattern and the distance between them with sub 100 nm resolution. Adhesion of primary neurons and directed neuronal outgrowth were observed on these protein patterns. Cellular constituents such as filopodia adhere to different printed protein elements of the discontinuous gradient including features as small as 75 nm.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000320002800019
DO  - DOI:10.7567/JJAP.52.05DA19
UR  - https://juser.fz-juelich.de/record/138443
ER  -