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000014281 0247_ $$2pmid$$apmid:21147989
000014281 0247_ $$2pmc$$apmc:PMC3393111
000014281 0247_ $$2DOI$$a10.1523/JNEUROSCI.4225-10.2010
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000014281 041__ $$aeng
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000014281 084__ $$2WoS$$aNeurosciences
000014281 1001_ $$0P:(DE-HGF)0$$aMobley, A.S.$$b0
000014281 245__ $$aHyperpolarization-activated cyclic nucleotide-gated channels in olfactory sensory neurons regulate axon extension and glomerular formation
000014281 260__ $$aWashington, DC$$bSoc.$$c2010
000014281 300__ $$a16498 - 16508
000014281 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000014281 3367_ $$2DataCite$$aOutput Types/Journal article
000014281 3367_ $$00$$2EndNote$$aJournal Article
000014281 3367_ $$2BibTeX$$aARTICLE
000014281 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000014281 3367_ $$2DRIVER$$aarticle
000014281 440_0 $$03603$$aJournal of Neuroscience$$v30$$x0270-6474$$y49
000014281 500__ $$aThis work was funded by National Institutes of Health (NIH)-National Institute on Deafness and Other Communication Disorders and NIH-National Institute on Aging grants (C.A.G.). A.M.M. was supported by Medical Scientist Training Program Grant GM07205 and NIH Grant F30 DC010324. A.S.M. was supported by Neurobiology Training Grant NS 007224-24 and National Research Service Award F32 DC010098-01A1. We thank Drs. L. Rela and M. B. Richard for developing the whole-mount imaging technique in our lab. HCN antibodies were generously provided by Dr. R. Shigemoto, National Institute for Physiological Sciences, Japan.
000014281 520__ $$aMechanisms influencing the development of olfactory bulb glomeruli are poorly understood. While odor receptors (ORs) play an important role in olfactory sensory neuron (OSN) axon targeting/coalescence (Mombaerts et al., 1996; Wang et al., 1998; Feinstein and Mombaerts, 2004), recent work showed that G protein activation alone is sufficient to induce OSN axon coalescence (Imai et al., 2006; Chesler et al., 2007), suggesting an activity-dependent mechanism in glomerular development. Consistent with these data, OSN axon projections and convergence are perturbed in mice deficient for adenylyl cyclase III, which is downstream from the OR and catalyzes the conversion of ATP to cAMP. However, in cyclic nucleotide-gated (CNG) channel knock-out mice OSN axons are only transiently perturbed (Lin et al., 2000), suggesting that the CNG channel may not be the sole target of cAMP. This prompted us to investigate an alternative channel, the hyperpolarization-activated, cyclic nucleotide-gated cation channel (HCN), as a potential developmental target of cAMP in OSNs. Here, we demonstrate that HCN channels are developmentally precocious in OSNs and therefore are plausible candidates for affecting OSN axon development. Inhibition of HCN channels in dissociated OSNs significantly reduced neurite outgrowth. Moreover, in HCN1 knock-out mice the formation of glomeruli was delayed in parallel with perturbations of axon organization in the olfactory nerve. These data support the hypothesis that the outgrowth and coalescence of OSN axons is, at least in part, subject to activity-dependent mechanisms mediated via HCN channels.
000014281 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000014281 588__ $$aDataset connected to Web of Science, Pubmed
000014281 650_2 $$2MeSH$$aAnimals
000014281 650_2 $$2MeSH$$aAnimals, Newborn
000014281 650_2 $$2MeSH$$aAntidiarrheals: pharmacology
000014281 650_2 $$2MeSH$$aAxons: drug effects
000014281 650_2 $$2MeSH$$aAxons: physiology
000014281 650_2 $$2MeSH$$aBiophysics: methods
000014281 650_2 $$2MeSH$$aCardiotonic Agents: pharmacology
000014281 650_2 $$2MeSH$$aCells, Cultured
000014281 650_2 $$2MeSH$$aCyclic Nucleotide-Gated Cation Channels: deficiency
000014281 650_2 $$2MeSH$$aCyclic Nucleotide-Gated Cation Channels: physiology
000014281 650_2 $$2MeSH$$aElectric Stimulation: methods
000014281 650_2 $$2MeSH$$aEmbryo, Mammalian
000014281 650_2 $$2MeSH$$aGAP-43 Protein: metabolism
000014281 650_2 $$2MeSH$$aGene Expression Regulation, Developmental: drug effects
000014281 650_2 $$2MeSH$$aGene Expression Regulation, Developmental: physiology
000014281 650_2 $$2MeSH$$aGreen Fluorescent Proteins: genetics
000014281 650_2 $$2MeSH$$aIon Channels: genetics
000014281 650_2 $$2MeSH$$aIon Channels: metabolism
000014281 650_2 $$2MeSH$$aLoperamide: pharmacology
000014281 650_2 $$2MeSH$$aMembrane Potentials: drug effects
000014281 650_2 $$2MeSH$$aMembrane Potentials: genetics
000014281 650_2 $$2MeSH$$aMice
000014281 650_2 $$2MeSH$$aMice, Inbred C57BL
000014281 650_2 $$2MeSH$$aMice, Knockout
000014281 650_2 $$2MeSH$$aNeural Cell Adhesion Molecules: metabolism
000014281 650_2 $$2MeSH$$aNeurogenesis: drug effects
000014281 650_2 $$2MeSH$$aNeurogenesis: physiology
000014281 650_2 $$2MeSH$$aOlfactory Bulb: cytology
000014281 650_2 $$2MeSH$$aOlfactory Bulb: embryology
000014281 650_2 $$2MeSH$$aOlfactory Bulb: growth & development
000014281 650_2 $$2MeSH$$aPatch-Clamp Techniques: methods
000014281 650_2 $$2MeSH$$aPotassium Channels: deficiency
000014281 650_2 $$2MeSH$$aPotassium Channels: genetics
000014281 650_2 $$2MeSH$$aPotassium Channels: metabolism
000014281 650_2 $$2MeSH$$aPotassium Channels: physiology
000014281 650_2 $$2MeSH$$aPyrimidines: pharmacology
000014281 650_2 $$2MeSH$$aReceptors, Odorant: genetics
000014281 650_2 $$2MeSH$$aReceptors, Odorant: metabolism
000014281 650_2 $$2MeSH$$aSensory Receptor Cells: cytology
000014281 650_2 $$2MeSH$$aSensory Receptor Cells: drug effects
000014281 650_7 $$00$$2NLM Chemicals$$aAntidiarrheals
000014281 650_7 $$00$$2NLM Chemicals$$aCardiotonic Agents
000014281 650_7 $$00$$2NLM Chemicals$$aCyclic Nucleotide-Gated Cation Channels
000014281 650_7 $$00$$2NLM Chemicals$$aGAP-43 Protein
000014281 650_7 $$00$$2NLM Chemicals$$aHCN2 potassium channel
000014281 650_7 $$00$$2NLM Chemicals$$aHCN4 protein, rat
000014281 650_7 $$00$$2NLM Chemicals$$aIon Channels
000014281 650_7 $$00$$2NLM Chemicals$$aNeural Cell Adhesion Molecules
000014281 650_7 $$00$$2NLM Chemicals$$aOlfr1507 protein, mouse
000014281 650_7 $$00$$2NLM Chemicals$$aPotassium Channels
000014281 650_7 $$00$$2NLM Chemicals$$aPyrimidines
000014281 650_7 $$00$$2NLM Chemicals$$aReceptors, Odorant
000014281 650_7 $$00$$2NLM Chemicals$$ahyperpolarization-activated cation channel
000014281 650_7 $$0133059-99-1$$2NLM Chemicals$$aICI D2788
000014281 650_7 $$0147336-22-9$$2NLM Chemicals$$aGreen Fluorescent Proteins
000014281 650_7 $$053179-11-6$$2NLM Chemicals$$aLoperamide
000014281 650_7 $$2WoSType$$aJ
000014281 7001_ $$0P:(DE-HGF)0$$aMiller, A.M.$$b1
000014281 7001_ $$0P:(DE-HGF)0$$aAraneda, R.C.$$b2
000014281 7001_ $$0P:(DE-HGF)0$$aMaurer, L.R.$$b3
000014281 7001_ $$0P:(DE-Juel1)131939$$aMüller, F.$$b4$$uFZJ
000014281 7001_ $$0P:(DE-HGF)0$$aGreer, C.A.$$b5
000014281 773__ $$0PERI:(DE-600)1475274-8$$a10.1523/JNEUROSCI.4225-10.2010$$gVol. 30, p. 16498 - 16508$$p16498 - 16508$$q30<16498 - 16508$$tThe @journal of neuroscience$$v30$$x0270-6474$$y2010
000014281 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3393111
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