Journal Article

PreJuSER-14281

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hyperpolarization-activated cyclic nucleotide-gated channels in olfactory sensory neurons regulate axon extension and glomerular formation

Mobley, A. S. ; Miller, A. M. ; Araneda, R. C. ; Maurer, L. R. ; Müller, F.FZJ* ; Greer, C. A.

2010
Soc. Washington, DC

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Please use a persistent id in citations: doi:10.1523/JNEUROSCI.4225-10.2010

Abstract: Mechanisms influencing the development of olfactory bulb glomeruli are poorly understood. While odor receptors (ORs) play an important role in olfactory sensory neuron (OSN) axon targeting/coalescence (Mombaerts et al., 1996; Wang et al., 1998; Feinstein and Mombaerts, 2004), recent work showed that G protein activation alone is sufficient to induce OSN axon coalescence (Imai et al., 2006; Chesler et al., 2007), suggesting an activity-dependent mechanism in glomerular development. Consistent with these data, OSN axon projections and convergence are perturbed in mice deficient for adenylyl cyclase III, which is downstream from the OR and catalyzes the conversion of ATP to cAMP. However, in cyclic nucleotide-gated (CNG) channel knock-out mice OSN axons are only transiently perturbed (Lin et al., 2000), suggesting that the CNG channel may not be the sole target of cAMP. This prompted us to investigate an alternative channel, the hyperpolarization-activated, cyclic nucleotide-gated cation channel (HCN), as a potential developmental target of cAMP in OSNs. Here, we demonstrate that HCN channels are developmentally precocious in OSNs and therefore are plausible candidates for affecting OSN axon development. Inhibition of HCN channels in dissociated OSNs significantly reduced neurite outgrowth. Moreover, in HCN1 knock-out mice the formation of glomeruli was delayed in parallel with perturbations of axon organization in the olfactory nerve. These data support the hypothesis that the outgrowth and coalescence of OSN axons is, at least in part, subject to activity-dependent mechanisms mediated via HCN channels.

Keyword(s): Animals (MeSH) ; Animals, Newborn (MeSH) ; Antidiarrheals: pharmacology (MeSH) ; Axons: drug effects (MeSH) ; Axons: physiology (MeSH) ; Biophysics: methods (MeSH) ; Cardiotonic Agents: pharmacology (MeSH) ; Cells, Cultured (MeSH) ; Cyclic Nucleotide-Gated Cation Channels: deficiency (MeSH) ; Cyclic Nucleotide-Gated Cation Channels: physiology (MeSH) ; Electric Stimulation: methods (MeSH) ; Embryo, Mammalian (MeSH) ; GAP-43 Protein: metabolism (MeSH) ; Gene Expression Regulation, Developmental: drug effects (MeSH) ; Gene Expression Regulation, Developmental: physiology (MeSH) ; Green Fluorescent Proteins: genetics (MeSH) ; Ion Channels: genetics (MeSH) ; Ion Channels: metabolism (MeSH) ; Loperamide: pharmacology (MeSH) ; Membrane Potentials: drug effects (MeSH) ; Membrane Potentials: genetics (MeSH) ; Mice (MeSH) ; Mice, Inbred C57BL (MeSH) ; Mice, Knockout (MeSH) ; Neural Cell Adhesion Molecules: metabolism (MeSH) ; Neurogenesis: drug effects (MeSH) ; Neurogenesis: physiology (MeSH) ; Olfactory Bulb: cytology (MeSH) ; Olfactory Bulb: embryology (MeSH) ; Olfactory Bulb: growth & development (MeSH) ; Patch-Clamp Techniques: methods (MeSH) ; Potassium Channels: deficiency (MeSH) ; Potassium Channels: genetics (MeSH) ; Potassium Channels: metabolism (MeSH) ; Potassium Channels: physiology (MeSH) ; Pyrimidines: pharmacology (MeSH) ; Receptors, Odorant: genetics (MeSH) ; Receptors, Odorant: metabolism (MeSH) ; Sensory Receptor Cells: cytology (MeSH) ; Sensory Receptor Cells: drug effects (MeSH) ; Antidiarrheals ; Cardiotonic Agents ; Cyclic Nucleotide-Gated Cation Channels ; GAP-43 Protein ; HCN2 potassium channel ; HCN4 protein, rat ; Ion Channels ; Neural Cell Adhesion Molecules ; Olfr1507 protein, mouse ; Potassium Channels ; Pyrimidines ; Receptors, Odorant ; hyperpolarization-activated cation channel ; ICI D2788 ; Green Fluorescent Proteins ; Loperamide ; J

Note: This work was funded by National Institutes of Health (NIH)-National Institute on Deafness and Other Communication Disorders and NIH-National Institute on Aging grants (C.A.G.). A.M.M. was supported by Medical Scientist Training Program Grant GM07205 and NIH Grant F30 DC010324. A.S.M. was supported by Neurobiology Training Grant NS 007224-24 and National Research Service Award F32 DC010098-01A1. We thank Drs. L. Rela and M. B. Richard for developing the whole-mount imaging technique in our lab. HCN antibodies were generously provided by Dr. R. Shigemoto, National Institute for Physiological Sciences, Japan.

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Contributing Institute(s):

1. Zelluläre Biophysik (ISB-1)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2010

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