Journal Article

PreJuSER-14601

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural characterization of intracellular C-terminal domains of group III metabotropic glutamate receptors

Seebahn, A. ; Dinkel, H. ; Mohrlüder, J.FZJ* ; Hartmann, R.FZJ* ; Vogel, N. ; Becker, C. M. ; Sticht, H. ; Enz, R.

2011
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.febslet.2010.12.042

Abstract: Metabotropic glutamate receptors (mGluRs) are regulated by interacting proteins that mostly bind to their intracellular C-termini. Here, we investigated if mGluR6, mGluR7a and mGluR8a C-termini form predefined binding surfaces or if they were rather unstructured. Limited tryptic digest of purified peptides argued against the formation of stable globular folds. Circular dichroism, (1)H NMR and (1)H(15)N HSQC spectra indicated the absence of rigid secondary structure elements. Furthermore, we localized short linear binding motifs in the unstructured receptor domains. Our data provide evidence that protein interactions of the analyzed mGluR C-termini are mediated rather by short linear motifs than by preformed folds.

Keyword(s): Amino Acid Motifs (MeSH) ; Animals (MeSH) ; Circular Dichroism (MeSH) ; Computational Biology: methods (MeSH) ; Nuclear Magnetic Resonance, Biomolecular (MeSH) ; Peptide Fragments: chemistry (MeSH) ; Peptide Fragments: metabolism (MeSH) ; Protein Folding (MeSH) ; Protein Hydrolysates: chemistry (MeSH) ; Protein Interaction Domains and Motifs (MeSH) ; Protein Isoforms: chemistry (MeSH) ; Protein Isoforms: metabolism (MeSH) ; Protein Structure, Secondary (MeSH) ; Rats (MeSH) ; Receptors, Metabotropic Glutamate: chemistry (MeSH) ; Receptors, Metabotropic Glutamate: genetics (MeSH) ; Receptors, Metabotropic Glutamate: metabolism (MeSH) ; Recombinant Fusion Proteins: chemistry (MeSH) ; Recombinant Fusion Proteins: metabolism (MeSH) ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH) ; Peptide Fragments ; Protein Hydrolysates ; Protein Isoforms ; Receptors, Metabotropic Glutamate ; Recombinant Fusion Proteins ; metabotropic glutamate receptor 6 ; metabotropic glutamate receptor 7 ; metabotropic glutamate receptor 8 ; J ; G-protein coupled receptor (auto) ; Metabotropic glutamate receptor (auto) ; Neurotransmitter receptor (auto) ; Short linear motif (auto)

Note: We thank Wei Xiang for help with mass spectrometry and Dieter Willbold for helpful discussions. This work was supported by the Deutsche Forschungsgemeinschaft [EN349/5-2, EU-HEALTH-F4-2008-202088, SFB539, SFB796].

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (P33)
2. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
3. NEUROCYPRES - Neurotransmitter Cys-loop receptors: structure, function and disease (202088) (202088)

Appears in the scientific report 2011

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