TY  - JOUR
AU  - Seebahn, A.
AU  - Dinkel, H.
AU  - Mohrlüder, J.
AU  - Hartmann, R.
AU  - Vogel, N.
AU  - Becker, C.M.
AU  - Sticht, H.
AU  - Enz, R.
TI  - Structural characterization of intracellular C-terminal domains of group III metabotropic glutamate receptors
JO  - FEBS letters
VL  - 585
SN  - 0014-5793
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PreJuSER-14601
SP  - 511 - 516
PY  - 2011
N1  - We thank Wei Xiang for help with mass spectrometry and Dieter Willbold for helpful discussions. This work was supported by the Deutsche Forschungsgemeinschaft [EN349/5-2, EU-HEALTH-F4-2008-202088, SFB539, SFB796].
AB  - Metabotropic glutamate receptors (mGluRs) are regulated by interacting proteins that mostly bind to their intracellular C-termini. Here, we investigated if mGluR6, mGluR7a and mGluR8a C-termini form predefined binding surfaces or if they were rather unstructured. Limited tryptic digest of purified peptides argued against the formation of stable globular folds. Circular dichroism, (1)H NMR and (1)H(15)N HSQC spectra indicated the absence of rigid secondary structure elements. Furthermore, we localized short linear binding motifs in the unstructured receptor domains. Our data provide evidence that protein interactions of the analyzed mGluR C-termini are mediated rather by short linear motifs than by preformed folds.
KW  - Amino Acid Motifs
KW  - Animals
KW  - Circular Dichroism
KW  - Computational Biology: methods
KW  - Nuclear Magnetic Resonance, Biomolecular
KW  - Peptide Fragments: chemistry
KW  - Peptide Fragments: metabolism
KW  - Protein Folding
KW  - Protein Hydrolysates: chemistry
KW  - Protein Interaction Domains and Motifs
KW  - Protein Isoforms: chemistry
KW  - Protein Isoforms: metabolism
KW  - Protein Structure, Secondary
KW  - Rats
KW  - Receptors, Metabotropic Glutamate: chemistry
KW  - Receptors, Metabotropic Glutamate: genetics
KW  - Receptors, Metabotropic Glutamate: metabolism
KW  - Recombinant Fusion Proteins: chemistry
KW  - Recombinant Fusion Proteins: metabolism
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
KW  - Peptide Fragments (NLM Chemicals)
KW  - Protein Hydrolysates (NLM Chemicals)
KW  - Protein Isoforms (NLM Chemicals)
KW  - Receptors, Metabotropic Glutamate (NLM Chemicals)
KW  - Recombinant Fusion Proteins (NLM Chemicals)
KW  - metabotropic glutamate receptor 6 (NLM Chemicals)
KW  - metabotropic glutamate receptor 7 (NLM Chemicals)
KW  - metabotropic glutamate receptor 8 (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21219903
UR  - <Go to ISI:>//WOS:000286657700011
DO  - DOI:10.1016/j.febslet.2010.12.042
UR  - https://juser.fz-juelich.de/record/14601
ER  -