TY  - JOUR
AU  - Cremer, C.M.
AU  - Lübke, J.H.R.
AU  - Palomero-Gallagher, N.
AU  - Zilles, K.
TI  - Laminar distribution of neurotransmitter receptors in different reeler mouse brain regions
JO  - Brain structure & function
VL  - 216
SN  - 1863-2653
CY  - Berlin
PB  - Springer
M1  - PreJuSER-14888
SP  - 201 - 218
PY  - 2011
N1  - The excellent technical assistance by S. Buller, M. Cremer and S. Wilms is very much appreciated. We further thank Dr. Axel Schleicher for helpful comments on the data, and Drs. Iris Hack and Astrid Rollenhagen for their critical reading and comments on the final version of the manuscript. This work was supported by the Initiative and Networking Fund of the Helmholtz Association (Helmholtz Alliance on Mental Health in an Ageing Society).
AB  - Mapping of multiple receptors of neurotransmitters provides insight into the spatial distribution of neurotransmission-relevant molecules in the cerebral cortex. During development, lack of reelin leads to impaired migration, disturbed lamination of the hippocampus and inverted neocortical layering. In the adult, reelin may regulate synaptic plasticity by modulating neurotransmitter receptor function. Using quantitative in vitro receptor autoradiography, different receptors, in particular, the binding site densities and laminar distribution of various glutamate, GABA, muscarinic and nicotinic acetylcholine, serotonin, dopamine and adenosine receptors, were analyzed in cortical and subcortical structures of reeler and wild-type brains. Differential changes in the laminar distribution, maximum binding capacity (B (max)) and regional density of neurotransmitter receptors were found in the reeler brain. A decrease of whole brain B (max) was found for adenosine A(1) and GABA(A) receptors. In the forebrain, several binding sites were differentially up- or down-regulated (kainate, A(1), benzodiazepine, 5-HT(1), M(2), α(1) and α(2)). In the hippocampus, a significant decrease of GABA(B), 5-HT(1) and A'₁ receptors were observed. The density of M(2) receptors increased, while other receptors remained unchanged. In the neocortex, some receptors demonstrated an obviously inverted laminar distribution (AMPA, kainate, NMDA, GABA(B), 5-HT(1), M(1), M(3), nAch), while the distribution of others (A(1), GABA(A), benzodiazepine, 5-HT(2), muscarinic M(2), adrenergic α(1), α(2)) seemed to be less affected. Thus, the laminar receptor distribution is modulated by the developmental impairment and suggests and reflects partially the laminar inversion in reeler mice.
KW  - Analysis of Variance
KW  - Animals
KW  - Autoradiography
KW  - Brain: anatomy & histology
KW  - Brain: metabolism
KW  - Brain Mapping: methods
KW  - Cell Adhesion Molecules, Neuronal: deficiency
KW  - Densitometry
KW  - Extracellular Matrix Proteins: deficiency
KW  - Image Processing, Computer-Assisted
KW  - Male
KW  - Mice
KW  - Mice, Neurologic Mutants: anatomy & histology
KW  - Mice, Neurologic Mutants: metabolism
KW  - Nerve Tissue Proteins: deficiency
KW  - Radioligand Assay
KW  - Receptors, Neurotransmitter: metabolism
KW  - Regression Analysis
KW  - Serine Endopeptidases: deficiency
KW  - Synaptic Transmission: physiology
KW  - Cell Adhesion Molecules, Neuronal (NLM Chemicals)
KW  - Extracellular Matrix Proteins (NLM Chemicals)
KW  - Nerve Tissue Proteins (NLM Chemicals)
KW  - Receptors, Neurotransmitter (NLM Chemicals)
KW  - Serine Endopeptidases (NLM Chemicals)
KW  - reelin protein (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21442415
UR  - <Go to ISI:>//WOS:000293924300004
DO  - DOI:10.1007/s00429-011-0303-3
UR  - https://juser.fz-juelich.de/record/14888
ER  -