Journal Article

PreJuSER-14888

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Laminar distribution of neurotransmitter receptors in different reeler mouse brain regions

Cremer, C. M.FZJ* ; Lübke, J. H. R.FZJ* ; Palomero-Gallagher, N.FZJ* ; Zilles, K.FZJ*

2011
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00429-011-0303-3

Abstract: Mapping of multiple receptors of neurotransmitters provides insight into the spatial distribution of neurotransmission-relevant molecules in the cerebral cortex. During development, lack of reelin leads to impaired migration, disturbed lamination of the hippocampus and inverted neocortical layering. In the adult, reelin may regulate synaptic plasticity by modulating neurotransmitter receptor function. Using quantitative in vitro receptor autoradiography, different receptors, in particular, the binding site densities and laminar distribution of various glutamate, GABA, muscarinic and nicotinic acetylcholine, serotonin, dopamine and adenosine receptors, were analyzed in cortical and subcortical structures of reeler and wild-type brains. Differential changes in the laminar distribution, maximum binding capacity (B (max)) and regional density of neurotransmitter receptors were found in the reeler brain. A decrease of whole brain B (max) was found for adenosine A(1) and GABA(A) receptors. In the forebrain, several binding sites were differentially up- or down-regulated (kainate, A(1), benzodiazepine, 5-HT(1), M(2), α(1) and α(2)). In the hippocampus, a significant decrease of GABA(B), 5-HT(1) and A'₁ receptors were observed. The density of M(2) receptors increased, while other receptors remained unchanged. In the neocortex, some receptors demonstrated an obviously inverted laminar distribution (AMPA, kainate, NMDA, GABA(B), 5-HT(1), M(1), M(3), nAch), while the distribution of others (A(1), GABA(A), benzodiazepine, 5-HT(2), muscarinic M(2), adrenergic α(1), α(2)) seemed to be less affected. Thus, the laminar receptor distribution is modulated by the developmental impairment and suggests and reflects partially the laminar inversion in reeler mice.

Keyword(s): Analysis of Variance (MeSH) ; Animals (MeSH) ; Autoradiography (MeSH) ; Brain: anatomy & histology (MeSH) ; Brain: metabolism (MeSH) ; Brain Mapping: methods (MeSH) ; Cell Adhesion Molecules, Neuronal: deficiency (MeSH) ; Densitometry (MeSH) ; Extracellular Matrix Proteins: deficiency (MeSH) ; Image Processing, Computer-Assisted (MeSH) ; Male (MeSH) ; Mice (MeSH) ; Mice, Neurologic Mutants: anatomy & histology (MeSH) ; Mice, Neurologic Mutants: metabolism (MeSH) ; Nerve Tissue Proteins: deficiency (MeSH) ; Radioligand Assay (MeSH) ; Receptors, Neurotransmitter: metabolism (MeSH) ; Regression Analysis (MeSH) ; Serine Endopeptidases: deficiency (MeSH) ; Synaptic Transmission: physiology (MeSH) ; Cell Adhesion Molecules, Neuronal ; Extracellular Matrix Proteins ; Nerve Tissue Proteins ; Receptors, Neurotransmitter ; Serine Endopeptidases ; reelin protein ; J ; Reeler mouse (auto) ; Quantitative receptor autoradiography (auto) ; Neurotransmitter receptor mapping (auto) ; Cortical organization (auto) ; Synaptic transmission and plasticity (auto)

Note: The excellent technical assistance by S. Buller, M. Cremer and S. Wilms is very much appreciated. We further thank Dr. Axel Schleicher for helpful comments on the data, and Drs. Iris Hack and Astrid Rollenhagen for their critical reading and comments on the final version of the manuscript. This work was supported by the Initiative and Networking Fund of the Helmholtz Association (Helmholtz Alliance on Mental Health in an Ageing Society).

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Contributing Institute(s):

1. Molekulare Organisation des Gehirns (INM-2)

Research Program(s):

1. Funktion und Dysfunktion des Nervensystems (FUEK409) (FUEK409)
2. 89571 - Connectivity and Activity (POF2-89571) (POF2-89571)

Appears in the scientific report 2011

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