Contribution to a conference proceedings

FZJ-2014-00638

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An application of a scanning light-addressable potentiometric sensor for label-free DNA detection

Wu, C. (Corresponding author)FZJ* ; Poghossian, A.FZJ* ; Werner, C. F. ; Bronder, T. ; Bäcker, M.FZJ* ; Schöning, M. J.FZJ* ; Wang, P.

2013
AMA Service GmbH, P.O. Box 2352, 31506 Wunstorf, Germany

This record in other databases:

Please use a persistent id in citations: doi:10.5162/11dss2013/A9

Abstract: In this study, a scanning light-addressable potentiometric sensor (LAPS) was applied for label-free and direct electrical detection of DNA (deoxyribonucleic acid) hybridization for the first time. The functioning principle of the sensor is based on an electrostatic detection of the intrinsic molecular charge of DNA molecules together with an ion-concentration redistribution within intermolecular spaces. The LAPS signal shows a distinct dependence on the concentration of the complementary target DNA. The mechanism of the shift of the photocurrent-voltage curve and the LAPS signal generation induced by the DNA immobilization and hybridization event is discussed.

---

Contributing Institute(s):

1. Bioelektronik (PGI-8)
2. JARA-FIT (JARA-FIT)
3. Bioelektronik (ICS-8)

Research Program(s):

1. 423 - Sensorics and bioinspired systems (POF2-423) (POF2-423)
2. 453 - Physics of the Cell (POF2-453) (POF2-453)

Appears in the scientific report 2013

---