000150606 001__ 150606
000150606 005__ 20240619091112.0
000150606 0247_ $$2doi$$a10.1016/j.jbiotec.2012.03.014
000150606 0247_ $$2ISSN$$a0168-1656
000150606 0247_ $$2ISSN$$a1873-4863
000150606 0247_ $$2WOS$$aWOS:000315156800004
000150606 0247_ $$2altmetric$$aaltmetric:699258
000150606 0247_ $$2pmid$$apmid:22465601
000150606 037__ $$aFZJ-2014-00655
000150606 082__ $$a540
000150606 1001_ $$0P:(DE-Juel1)128668$$aBäcker, M.$$b0$$eCorresponding author$$ufzj
000150606 245__ $$aChip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis.
000150606 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2013
000150606 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1390919971_7661
000150606 3367_ $$2DataCite$$aOutput Types/Journal article
000150606 3367_ $$00$$2EndNote$$aJournal Article
000150606 3367_ $$2BibTeX$$aARTICLE
000150606 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000150606 3367_ $$2DRIVER$$aarticle
000150606 500__ $$3POF3_Assignment on 2016-02-29
000150606 520__ $$aA microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
000150606 536__ $$0G:(DE-HGF)POF2-423$$a423 - Sensorics and bioinspired systems (POF2-423)$$cPOF2-423$$fPOF II$$x0
000150606 536__ $$0G:(DE-HGF)POF2-453$$a453 - Physics of the Cell (POF2-453)$$cPOF2-453$$fPOF II$$x1
000150606 588__ $$aDataset connected to CrossRef, juser.fz-juelich.de
000150606 7001_ $$0P:(DE-HGF)0$$aRakowski, D.$$b1
000150606 7001_ $$0P:(DE-Juel1)128717$$aPoghossian, A.$$b2$$ufzj
000150606 7001_ $$0P:(DE-HGF)0$$aBiselli, M.$$b3
000150606 7001_ $$0P:(DE-HGF)0$$aWagner, P.$$b4
000150606 7001_ $$0P:(DE-Juel1)128727$$aSchöning, M. J.$$b5$$ufzj
000150606 773__ $$0PERI:(DE-600)2016476-2$$a10.1016/j.jbiotec.2012.03.014$$gVol. 163, no. 4, p. 371 - 376$$n4$$p371 - 376$$tJournal of biotechnology$$v163$$x0168-1656$$y2013
000150606 8564_ $$uhttps://juser.fz-juelich.de/record/150606/files/FZJ-2014-00655.pdf$$yRestricted$$zPublished final document.
000150606 909CO $$ooai:juser.fz-juelich.de:150606$$pVDB
000150606 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128668$$aForschungszentrum Jülich GmbH$$b0$$kFZJ
000150606 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128717$$aForschungszentrum Jülich GmbH$$b2$$kFZJ
000150606 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128727$$aForschungszentrum Jülich GmbH$$b5$$kFZJ
000150606 9132_ $$0G:(DE-HGF)POF3-559H$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vAddenda$$x0
000150606 9132_ $$0G:(DE-HGF)POF3-529H$$1G:(DE-HGF)POF3-520$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lFuture Information Technology - Fundamentals, Novel Concepts and Energy Efficiency (FIT)$$vAddenda$$x1
000150606 9131_ $$0G:(DE-HGF)POF2-423$$1G:(DE-HGF)POF2-420$$2G:(DE-HGF)POF2-400$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$aDE-HGF$$bSchlüsseltechnologien$$lGrundlagen zukünftiger Informationstechnologien$$vSensorics and bioinspired systems$$x0
000150606 9131_ $$0G:(DE-HGF)POF2-453$$1G:(DE-HGF)POF2-450$$2G:(DE-HGF)POF2-400$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$aDE-HGF$$bSchlüsseltechnologien$$lBioSoft$$vPhysics of the Cell$$x1
000150606 9141_ $$y2013
000150606 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000150606 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000150606 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000150606 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000150606 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000150606 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000150606 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000150606 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000150606 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000150606 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000150606 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000150606 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000150606 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences
000150606 9201_ $$0I:(DE-Juel1)PGI-8-20110106$$kPGI-8$$lBioelektronik$$x0
000150606 9201_ $$0I:(DE-82)080009_20140620$$kJARA-FIT$$lJARA-FIT$$x1
000150606 9201_ $$0I:(DE-Juel1)ICS-8-20110106$$kICS-8$$lBioelektronik$$x2
000150606 980__ $$ajournal
000150606 980__ $$aVDB
000150606 980__ $$aUNRESTRICTED
000150606 980__ $$aI:(DE-Juel1)PGI-8-20110106
000150606 980__ $$aI:(DE-82)080009_20140620
000150606 980__ $$aI:(DE-Juel1)ICS-8-20110106
000150606 981__ $$aI:(DE-Juel1)IBI-3-20200312
000150606 981__ $$aI:(DE-Juel1)ICS-8-20110106