Journal Article

FZJ-2014-00655

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis.

Bäcker, M. (Corresponding author)FZJ* ; Rakowski, D. ; Poghossian, A.FZJ* ; Biselli, M. ; Wagner, P. ; Schöning, M. J.FZJ*

2013
Elsevier Science Amsterdam [u.a.]

This record in other databases:      

Please use a persistent id in citations: doi:10.1016/j.jbiotec.2012.03.014

Abstract: A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.

---

Contributing Institute(s):

1. Bioelektronik (PGI-8)
2. JARA-FIT (JARA-FIT)
3. Bioelektronik (ICS-8)

Research Program(s):

1. 423 - Sensorics and bioinspired systems (POF2-423) (POF2-423)
2. 453 - Physics of the Cell (POF2-453) (POF2-453)

Appears in the scientific report 2013

---

Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---