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@ARTICLE{Bcker:150606,
      author       = {Bäcker, M. and Rakowski, D. and Poghossian, A. and
                      Biselli, M. and Wagner, P. and Schöning, M. J.},
      title        = {{C}hip-based amperometric enzyme sensor system for
                      monitoring of bioprocesses by flow-injection analysis.},
      journal      = {Journal of biotechnology},
      volume       = {163},
      number       = {4},
      issn         = {0168-1656},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2014-00655},
      pages        = {371 - 376},
      year         = {2013},
      abstract     = {A microfluidic chip integrating amperometric enzyme sensors
                      for the detection of glucose, glutamate and glutamine in
                      cell-culture fermentation processes has been developed. The
                      enzymes glucose oxidase, glutamate oxidase and glutaminase
                      were immobilized by means of cross-linking with
                      glutaraldehyde on platinum thin-film electrodes integrated
                      within a microfluidic channel. The biosensor chip was
                      coupled to a flow-injection analysis system for
                      electrochemical characterization of the sensors. The sensors
                      have been characterized in terms of sensitivity, linear
                      working range and detection limit. The sensitivity evaluated
                      from the respective peak areas was 1.47, 3.68 and 0.28
                      μAs/mM for the glucose, glutamate and glutamine sensor,
                      respectively. The calibration curves were linear up to a
                      concentration of 20 mM glucose and glutamine and up to 10 mM
                      for glutamate. The lower detection limit amounted to be 0.05
                      mM for the glucose and glutamate sensor, respectively, and
                      0.1 mM for the glutamine sensor. Experiments in cell-culture
                      medium have demonstrated a good correlation between the
                      glutamate, glutamine and glucose concentrations measured
                      with the chip-based biosensors in a differential-mode and
                      the commercially available instrumentation. The obtained
                      results demonstrate the feasibility of the realized
                      microfluidic biosensor chip for monitoring of bioprocesses.},
      cin          = {PGI-8 / JARA-FIT / ICS-8},
      ddc          = {540},
      cid          = {I:(DE-Juel1)PGI-8-20110106 / $I:(DE-82)080009_20140620$ /
                      I:(DE-Juel1)ICS-8-20110106},
      pnm          = {423 - Sensorics and bioinspired systems (POF2-423) / 453 -
                      Physics of the Cell (POF2-453)},
      pid          = {G:(DE-HGF)POF2-423 / G:(DE-HGF)POF2-453},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000315156800004},
      pubmed       = {pmid:22465601},
      doi          = {10.1016/j.jbiotec.2012.03.014},
      url          = {https://juser.fz-juelich.de/record/150606},
}