Journal Article

FZJ-2014-01178

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Single mimivirus particles intercepted and imaged with an X-ray laser

Seibert, M. M. (Corresponding author) ; Ekeberg, T. ; Maia, F. R. N. C. ; Svenda, M. ; Andreasson, J. ; Jönsson, O. ; Odić, D. ; Iwan, B. ; Rocker, A. ; Westphal, D. ; Hantke, M. ; DePonte, D. P. ; Barty, A. ; Schulz, J. ; Gumprecht, L. ; Coppola, N. ; Aquila, A. ; Liang, M. ; White, T. A. ; Martin, A. ; Caleman, C. ; Stern, S. ; Abergel, C. ; Seltzer, V. ; Claverie, J.-M. ; Bostedt, C. ; Bozek, J. D. ; Boutet, S. ; Miahnahri, A. A. ; Messerschmidt, M. ; Krzywinski, J. ; Williams, G. ; Hodgson, K. O. ; Bogan, M. J. ; Hampton, C. Y. ; Sierra, R. G. ; Starodub, D. ; Andersson, I. ; Bajt, S. ; Barthelmess, M. ; Spence, J. C. H. ; Fromme, P. ; Weierstall, U. ; Kirian, R. ; Hunter, M. ; Doak, R. B. ; Marchesini, S. ; Hau-Riege, S. P. ; Frank, M. ; Shoeman, R. L. ; Lomb, L. ; Epp, S. W. ; Hartmann, R. ; Rolles, D. ; Rudenko, A. ; Schmidt, C. ; Foucar, L. ; Kimmel, N. ; Holl, P. ; Rudek, B. ; Erk, B. ; Hömke, A. ; Reich, C. ; Pietschner, D. ; Weidenspointner, G. ; Strüder, L. ; Hauser, G. ; Gorke, H.FZJ* ; Ullrich, J. ; Schlichting, I. ; Herrmann, S. ; Schaller, G. ; Schopper, F. ; Soltau, H. ; Kühnel, K.-U. ; Andritschke, R. ; Schröter, C.-D. ; Krasniqi, F. ; Bott, M. ; Schorb, S. ; Rupp, D. ; Adolph, M. ; Gorkhover, T. ; Hirsemann, H. ; Potdevin, G. ; Graafsma, H. ; Nilsson, B. ; Chapman, H. N. ; Hajdu, J.

2011
Nature Publising Group78092 London [u.a.]

This record in other databases:      

Please use a persistent id in citations: doi:10.1038/nature09748

Abstract: X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions1, 2, 3, 4. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma1. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval2. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source5. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

---

Contributing Institute(s):

1. Zentralinstitut für Elektronik (ZEA-2)

Research Program(s):

1. 531 - Hadron Structure and Dynamics (HSD) (POF2-531) (POF2-531)

Appears in the scientific report 2014

---

Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

---