%0 Journal Article
%A Kroll, Tina
%A Elmenhorst, David
%A Weißhaupt, Angela
%A Beer, Simone
%A Bauer, Andreas
%T Reproducibility of Non-Invasive A1 Adenosine Receptor Quantification in the Rat Brain Using [18F]CPFPX and Positron Emission Tomography
%J Molecular imaging & biology
%V 16
%N 5
%@ 1536-1632
%C Amsterdam [u.a.]
%I Elsevier Science
%M FZJ-2014-01663
%P 699-709
%D 2014
%X PurposeThe A1AR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([18F]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A1 adenosine receptor (A1AR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal A1AR studies with [18F]CPFPX and a dedicated small animal positron emission tomography (PET) scanner.ProceduresTwelve male Sprague Dawley rats underwent four repeated dynamic PET scans with a bolus injection of [18F]CPFPX. A1AR availability was determined by different non-invasive approaches including simplified and multilinear reference tissue (olfactory bulb)-based models and graphical methods. The outcome parameter binding potential (BP) was evaluated in terms of variability and reproducibility.ResultsRepeated estimations of [18F]CPFPX BP ND gave reliable results with acceptable variability (mean 12 %) and reproducibility (intraclass correlation coefficients raging from 0.57 to 0.68) in cortical and subcortical regions of the rat brain. With regard to kinetic models, test-retest stability of the simplified reference-tissue model (SRTM) was superior to multilinear and graphical approaches.ConclusionsNon-invasive quantification of A1AR density in the rat brain is reproducible and reliable with [18F]CPFPX PET and allows longitudinal designs of in vivo imaging studies in rodents.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000342135800014
%R 10.1007/s11307-014-0729-0
%U https://juser.fz-juelich.de/record/151781