Journal Article

FZJ-2014-01663

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Reproducibility of Non-Invasive A1 Adenosine Receptor Quantification in the Rat Brain Using [18F]CPFPX and Positron Emission Tomography

Kroll, T. (Corresponding author)FZJ* ; Elmenhorst, D.FZJ* ; Weißhaupt, A.FZJ* ; Beer, S.FZJ* ; Bauer, A.FZJ*

2014
Elsevier Science Amsterdam [u.a.]

This record in other databases:  

Please use a persistent id in citations: doi:10.1007/s11307-014-0729-0

Abstract: PurposeThe A1AR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([18F]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A1 adenosine receptor (A1AR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal A1AR studies with [18F]CPFPX and a dedicated small animal positron emission tomography (PET) scanner.ProceduresTwelve male Sprague Dawley rats underwent four repeated dynamic PET scans with a bolus injection of [18F]CPFPX. A1AR availability was determined by different non-invasive approaches including simplified and multilinear reference tissue (olfactory bulb)-based models and graphical methods. The outcome parameter binding potential (BP) was evaluated in terms of variability and reproducibility.ResultsRepeated estimations of [18F]CPFPX BP ND gave reliable results with acceptable variability (mean 12 %) and reproducibility (intraclass correlation coefficients raging from 0.57 to 0.68) in cortical and subcortical regions of the rat brain. With regard to kinetic models, test-retest stability of the simplified reference-tissue model (SRTM) was superior to multilinear and graphical approaches.ConclusionsNon-invasive quantification of A1AR density in the rat brain is reproducible and reliable with [18F]CPFPX PET and allows longitudinal designs of in vivo imaging studies in rodents.

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Contributing Institute(s):

1. Molekulare Organisation des Gehirns (INM-2)

Research Program(s):

1. 333 - Pathophysiological Mechanisms of Neurological and Psychiatric Diseases (POF2-333) (POF2-333)
2. 89571 - Connectivity and Activity (POF2-89571) (POF2-89571)

Appears in the scientific report 2014

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Database coverage:
; Current Contents - Clinical Medicine ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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