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000015317 0247_ $$2DOI$$a10.1007/s00249-011-0689-0
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000015317 084__ $$2WoS$$aBiophysics
000015317 1001_ $$0P:(DE-HGF)0$$aWellert, S.$$b0
000015317 245__ $$aThe DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions: structure, dynamics, and enzyme activity
000015317 260__ $$aBerlin$$bSpringer$$c2011
000015317 300__ $$a761 - 774
000015317 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000015317 3367_ $$2DRIVER$$aarticle
000015317 440_0 $$010441$$aEuropean Biophysics Journal : with Biophysics Letters$$v40$$x0175-7571$$y6
000015317 500__ $$aJ.G. and M.M.B. were supported by the German Ministry of Defense under contract E/UR3G/6G115/6A801. T. H., R. S., C. S. also were financed by the German Ministry of Defense (Contracts E/E590/9Z004/5F159 and E/E590/8Z002/4F170). The SANS experiments were supported by the European Union through the NMI3 program. We are grateful to the Laboratoire Leon Brillouin and the Julich Center for Neutron Scattering at the FRM II for providing beamtime.
000015317 520__ $$aThe enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.
000015317 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000015317 536__ $$0G:(DE-Juel1)FUEK415$$2G:(DE-HGF)$$aGroßgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI)$$cP55$$x1
000015317 588__ $$aDataset connected to Web of Science, Pubmed
000015317 65320 $$2Author$$aDynamic light scattering
000015317 65320 $$2Author$$aNeutron spin echo
000015317 65320 $$2Author$$aMicroemulsion
000015317 65320 $$2Author$$aEnzyme catalysis
000015317 65320 $$2Author$$aSANS
000015317 65320 $$2Author$$aProtein structure
000015317 650_2 $$2MeSH$$aAnimals
000015317 650_2 $$2MeSH$$aCarbohydrates: chemistry
000015317 650_2 $$2MeSH$$aCarbohydrates: pharmacology
000015317 650_2 $$2MeSH$$aEmulsions
000015317 650_2 $$2MeSH$$aLoligo: enzymology
000015317 650_2 $$2MeSH$$aLoligo: metabolism
000015317 650_2 $$2MeSH$$aMicroscopy, Electron, Scanning
000015317 650_2 $$2MeSH$$aNeutron Diffraction
000015317 650_2 $$2MeSH$$aPhosphoric Triester Hydrolases: analysis
000015317 650_2 $$2MeSH$$aPhosphoric Triester Hydrolases: chemistry
000015317 650_2 $$2MeSH$$aPhosphoric Triester Hydrolases: metabolism
000015317 650_2 $$2MeSH$$aScattering, Small Angle
000015317 650_2 $$2MeSH$$aSolutions: chemistry
000015317 650_2 $$2MeSH$$aSurface-Active Agents: chemistry
000015317 650_2 $$2MeSH$$aSurface-Active Agents: pharmacology
000015317 650_7 $$00$$2NLM Chemicals$$aCarbohydrates
000015317 650_7 $$00$$2NLM Chemicals$$aEmulsions
000015317 650_7 $$00$$2NLM Chemicals$$aSolutions
000015317 650_7 $$00$$2NLM Chemicals$$aSurface-Active Agents
000015317 650_7 $$0EC 3.1.8.-$$2NLM Chemicals$$aPhosphoric Triester Hydrolases
000015317 650_7 $$0EC 3.1.8.2$$2NLM Chemicals$$adiisopropyl-fluorophosphatase
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000015317 693__ $$0EXP:(DE-MLZ)J-NSE-20140101$$1EXP:(DE-MLZ)FRMII-20140101$$5EXP:(DE-MLZ)J-NSE-20140101$$6EXP:(DE-MLZ)NL2ao-20140101$$aForschungs-Neutronenquelle Heinz Maier-Leibnitz$$eJ-NSE: Neutron spin-echo spectrometer$$fNL2ao$$x0
000015317 7001_ $$0P:(DE-HGF)0$$aTiersch, B.$$b1
000015317 7001_ $$0P:(DE-HGF)0$$aKoetz, J.$$b2
000015317 7001_ $$0P:(DE-HGF)0$$aRichardt, A.$$b3
000015317 7001_ $$0P:(DE-HGF)0$$aLapp, A.$$b4
000015317 7001_ $$0P:(DE-Juel1)130718$$aHolderer, O.$$b5$$uFZJ
000015317 7001_ $$0P:(DE-HGF)0$$aGäb, J.$$b6
000015317 7001_ $$0P:(DE-HGF)0$$aBlum, M.-M.$$b7
000015317 7001_ $$0P:(DE-HGF)0$$aSchulreich, C.$$b8
000015317 7001_ $$0P:(DE-HGF)0$$aStehle, R.$$b9
000015317 7001_ $$0P:(DE-HGF)0$$aHellweg, T.$$b10
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000015317 8567_ $$uhttp://dx.doi.org/10.1007/s00249-011-0689-0
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