Journal Article

PreJuSER-15317

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions: structure, dynamics, and enzyme activity

Wellert, S. ; Tiersch, B. ; Koetz, J. ; Richardt, A. ; Lapp, A. ; Holderer, O.FZJ* ; Gäb, J. ; Blum, M.-M. ; Schulreich, C. ; Stehle, R. ; Hellweg, T.

2011
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00249-011-0689-0

Abstract: The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.

Keyword(s): Animals (MeSH) ; Carbohydrates: chemistry (MeSH) ; Carbohydrates: pharmacology (MeSH) ; Emulsions (MeSH) ; Loligo: enzymology (MeSH) ; Loligo: metabolism (MeSH) ; Microscopy, Electron, Scanning (MeSH) ; Neutron Diffraction (MeSH) ; Phosphoric Triester Hydrolases: analysis (MeSH) ; Phosphoric Triester Hydrolases: chemistry (MeSH) ; Phosphoric Triester Hydrolases: metabolism (MeSH) ; Scattering, Small Angle (MeSH) ; Solutions: chemistry (MeSH) ; Surface-Active Agents: chemistry (MeSH) ; Surface-Active Agents: pharmacology (MeSH) ; Health and Life (1st) ; Health and Life (1st) ; Biology (2nd) ; Carbohydrates ; Emulsions ; Solutions ; Surface-Active Agents ; Phosphoric Triester Hydrolases ; diisopropyl-fluorophosphatase ; J ; Dynamic light scattering (auto) ; Neutron spin echo (auto) ; Microemulsion (auto) ; Enzyme catalysis (auto) ; SANS (auto) ; Protein structure (auto)

Note: J.G. and M.M.B. were supported by the German Ministry of Defense under contract E/UR3G/6G115/6A801. T. H., R. S., C. S. also were financed by the German Ministry of Defense (Contracts E/E590/9Z004/5F159 and E/E590/8Z002/4F170). The SANS experiments were supported by the European Union through the NMI3 program. We are grateful to the Laboratoire Leon Brillouin and the Julich Center for Neutron Scattering at the FRM II for providing beamtime.

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Contributing Institute(s):

1. Neutronenstreuung (ICS-1)
2. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
3. Neutronenstreuung (JCNS-1)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
2. Großgeräte für die Forschung mit Photonen, Neutronen und Ionen (PNI) (P55)

Experiment(s):

1. J-NSE: Neutron spin-echo spectrometer (NL2ao)

Appears in the scientific report 2011

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