TY  - JOUR
AU  - Wellert, S.
AU  - Tiersch, B.
AU  - Koetz, J.
AU  - Richardt, A.
AU  - Lapp, A.
AU  - Holderer, O.
AU  - Gäb, J.
AU  - Blum, M.-M.
AU  - Schulreich, C.
AU  - Stehle, R.
AU  - Hellweg, T.
TI  - The DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions: structure, dynamics, and enzyme activity
JO  - European biophysics journal
VL  - 40
SN  - 0175-7571
CY  - Berlin
PB  - Springer
M1  - PreJuSER-15317
SP  - 761 - 774
PY  - 2011
N1  - J.G. and M.M.B. were supported by the German Ministry of Defense under contract E/UR3G/6G115/6A801. T. H., R. S., C. S. also were financed by the German Ministry of Defense (Contracts E/E590/9Z004/5F159 and E/E590/8Z002/4F170). The SANS experiments were supported by the European Union through the NMI3 program. We are grateful to the Laboratoire Leon Brillouin and the Julich Center for Neutron Scattering at the FRM II for providing beamtime.
AB  - The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.
KW  - Animals
KW  - Carbohydrates: chemistry
KW  - Carbohydrates: pharmacology
KW  - Emulsions
KW  - Loligo: enzymology
KW  - Loligo: metabolism
KW  - Microscopy, Electron, Scanning
KW  - Neutron Diffraction
KW  - Phosphoric Triester Hydrolases: analysis
KW  - Phosphoric Triester Hydrolases: chemistry
KW  - Phosphoric Triester Hydrolases: metabolism
KW  - Scattering, Small Angle
KW  - Solutions: chemistry
KW  - Surface-Active Agents: chemistry
KW  - Surface-Active Agents: pharmacology
KW  - Carbohydrates (NLM Chemicals)
KW  - Emulsions (NLM Chemicals)
KW  - Solutions (NLM Chemicals)
KW  - Surface-Active Agents (NLM Chemicals)
KW  - Phosphoric Triester Hydrolases (NLM Chemicals)
KW  - diisopropyl-fluorophosphatase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21416312
UR  - <Go to ISI:>//WOS:000290331400006
DO  - DOI:10.1007/s00249-011-0689-0
UR  - https://juser.fz-juelich.de/record/15317
ER  -