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@ARTICLE{Rocco:153366,
      author       = {Rocco, Mattia and Molteni, Matteo and Ponassi, Marco and
                      Giachi, Guido and Frediani, Marco and Koutsioumpas,
                      Alexandros and Profumo, Aldo and Trevarin, Didier and
                      Cardinali, Barbara and Vachette, Patrice and Ferri, Fabio
                      and Pérez, Javier},
      title        = {{A} {C}omprehensive {M}echanism of {F}ibrin {N}etwork
                      {F}ormation {I}nvolving {E}arly {B}ranching and {D}elayed
                      {S}ingle- to {D}ouble-{S}trand {T}ransition from {C}oupled
                      {T}ime-{R}esolved {X}-ray/{L}ight-{S}cattering {D}etection},
      journal      = {Journal of the American Chemical Society},
      volume       = {136},
      number       = {14},
      issn         = {1520-5126},
      address      = {Washington, DC},
      publisher    = {American Chemical Society},
      reportid     = {FZJ-2014-02997},
      pages        = {5376 - 5384},
      year         = {2014},
      abstract     = {The formation of a fibrin network following fibrinogen
                      enzymatic activation is the central event in blood
                      coagulation and has important biomedical and
                      biotechnological implications. A non-covalent polymerization
                      reaction between macromolecular monomers, it consists
                      basically of two complementary processes:
                      elongation/branching generates an interconnected 3D scaffold
                      of relatively thin fibrils, and cooperative lateral
                      aggregation thickens them more than 10-fold. We have studied
                      the early stages up to the gel point by fast
                      fibrinogen:enzyme mixing experiments using simultaneous
                      small-angle X-ray scattering and wide-angle, multi-angle
                      light scattering detection. The coupled evolutions of the
                      average molecular weight, size, and cross section of the
                      solutes during the fibrils growth phase were thus recovered.
                      They reveal that extended structures, thinner than those
                      predicted by the classic half-staggered, double-stranded
                      mechanism, must quickly form. Following extensive modeling,
                      an initial phase is proposed in which single-bonded
                      “Y-ladder” polymers rapidly elongate before undergoing a
                      delayed transition to the double-stranded fibrils.
                      Consistent with the data, this alternative mechanism can
                      intrinsically generate frequent, random branching points in
                      each growing fibril. The model predicts that, as a
                      consequence, some branches in these expanding “lumps”
                      eventually interconnect, forming the pervasive 3D network.
                      While still growing, other branches will then undergo a
                      Ca2+/length-dependent cooperative collapse on the resulting
                      network scaffolding filaments, explaining their sudden
                      thickening, low final density, and basic mechanical
                      properties},
      cin          = {Neutronenstreuung ; JCNS-1 / JCNS (München) ; Jülich
                      Centre for Neutron Science JCNS (München) ; JCNS-FRM-II /
                      ICS-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)JCNS-1-20110106 /
                      I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)ICS-1-20110106},
      pnm          = {451 - Soft Matter Composites (POF2-451) / 54G - JCNS
                      (POF2-54G24)},
      pid          = {G:(DE-HGF)POF2-451 / G:(DE-HGF)POF2-54G24},
      experiment   = {EXP:(DE-MLZ)External-20140101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000334572200039},
      doi          = {10.1021/ja5002955},
      url          = {https://juser.fz-juelich.de/record/153366},
}