%0 Journal Article
%A Binder, Dennis
%A Grünberger, Alexander
%A Loeschcke, Anita
%A Probst, Christopher
%A Bier, Claus
%A Pietruszka, Jörg
%A Wiechert, Wolfgang
%A Kohlheyer, Dietrich
%A Jaeger, Karl-Erich
%A Drepper, Thomas
%T Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
%J Integrative biology
%V 6
%N 8
%@ 1757-9708
%C Cambridge
%I RSC Publ.
%M FZJ-2014-03427
%P 755-765
%D 2014
%X Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000339930500003
%$ pmid:24894989
%R 10.1039/c4ib00027g
%U https://juser.fz-juelich.de/record/153998