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000153998 0247_ $$2ISSN$$a1757-9708
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000153998 1001_ $$0P:(DE-Juel1)151178$$aBinder, Dennis$$b0
000153998 245__ $$aLight-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
000153998 260__ $$aCambridge$$bRSC Publ.$$c2014
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000153998 520__ $$aLight can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level.
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000153998 7001_ $$0P:(DE-Juel1)143612$$aGrünberger, Alexander$$b1
000153998 7001_ $$0P:(DE-Juel1)131500$$aLoeschcke, Anita$$b2
000153998 7001_ $$0P:(DE-Juel1)142191$$aProbst, Christopher$$b3
000153998 7001_ $$0P:(DE-Juel1)151164$$aBier, Claus$$b4
000153998 7001_ $$0P:(DE-Juel1)128906$$aPietruszka, Jörg$$b5
000153998 7001_ $$0P:(DE-Juel1)129076$$aWiechert, Wolfgang$$b6
000153998 7001_ $$0P:(DE-Juel1)140195$$aKohlheyer, Dietrich$$b7
000153998 7001_ $$0P:(DE-Juel1)131457$$aJaeger, Karl-Erich$$b8$$eCorresponding Author
000153998 7001_ $$0P:(DE-Juel1)131426$$aDrepper, Thomas$$b9
000153998 773__ $$0PERI:(DE-600)2480063-6$$a10.1039/c4ib00027g$$gp. 10.1039.c4ib00027g$$n8$$p755-765$$tIntegrative biology$$v6$$x1757-9708$$y2014
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