TY  - JOUR
AU  - Binder, Dennis
AU  - Grünberger, Alexander
AU  - Loeschcke, Anita
AU  - Probst, Christopher
AU  - Bier, Claus
AU  - Pietruszka, Jörg
AU  - Wiechert, Wolfgang
AU  - Kohlheyer, Dietrich
AU  - Jaeger, Karl-Erich
AU  - Drepper, Thomas
TI  - Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
JO  - Integrative biology
VL  - 6
IS  - 8
SN  - 1757-9708
CY  - Cambridge
PB  - RSC Publ.
M1  - FZJ-2014-03427
SP  - 755-765
PY  - 2014
AB  - Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000339930500003
C6  - pmid:24894989
DO  - DOI:10.1039/c4ib00027g
UR  - https://juser.fz-juelich.de/record/153998
ER  -