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@ARTICLE{Binder:153998,
      author       = {Binder, Dennis and Grünberger, Alexander and Loeschcke,
                      Anita and Probst, Christopher and Bier, Claus and
                      Pietruszka, Jörg and Wiechert, Wolfgang and Kohlheyer,
                      Dietrich and Jaeger, Karl-Erich and Drepper, Thomas},
      title        = {{L}ight-responsive control of bacterial gene {E}xpression:
                      precise triggering of the lac promoter activity using
                      photocaged {IPTG}},
      journal      = {Integrative biology},
      volume       = {6},
      number       = {8},
      issn         = {1757-9708},
      address      = {Cambridge},
      publisher    = {RSC Publ.},
      reportid     = {FZJ-2014-03427},
      pages        = {755-765},
      year         = {2014},
      abstract     = {Light can be used to control numerous cellular processes
                      including protein function and interaction as well as gene
                      expression in a non-invasive fashion and with unprecedented
                      spatiotemporal resolution. However, for chemical
                      phototriggers tight, gradual, and homogeneous light response
                      has never been attained in living cells. Here, we report on
                      a light-responsive bacterial T7 RNA polymerase expression
                      system based on a photocaged derivative of the inducer
                      molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We
                      have comparatively analyzed different Escherichia coli lac
                      promoter-regulated expression systems in batch and
                      microfluidic single-cell cultivation. The lacY-deficient E.
                      coli strain Tuner(DE3) harboring additional plasmid-born
                      copies of the lacI gene exhibited a sensitive and defined
                      response to increasing IPTG concentrations. Photocaged IPTG
                      served as a synthetic photo-switch to convert the E. coli
                      system into an optogenetic expression module allowing for
                      precise and gradual light-triggering of gene expression as
                      demonstrated at the single cell level.},
      cin          = {IBG-1 / IBOC},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IBOC-20090406},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000339930500003},
      pubmed       = {pmid:24894989},
      doi          = {10.1039/c4ib00027g},
      url          = {https://juser.fz-juelich.de/record/153998},
}