Home > Publications database > Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG > print

001     153998
005     20210129213823.0
024 7 _ |a 10.1039/c4ib00027g
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024 7 _ |a 1757-9694
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024 7 _ |a 1757-9708
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037 _ _ |a FZJ-2014-03427
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100 1 _ |0 P:(DE-Juel1)151178
|a Binder, Dennis
|b 0
245 _ _ |a Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
260 _ _ |a Cambridge
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|c 2014
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520 _ _ |a Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level.
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|a Grünberger, Alexander
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|a Loeschcke, Anita
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|a Probst, Christopher
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|a Bier, Claus
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|a Pietruszka, Jörg
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700 1 _ |0 P:(DE-Juel1)129076
|a Wiechert, Wolfgang
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|a Kohlheyer, Dietrich
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700 1 _ |0 P:(DE-Juel1)131457
|a Jaeger, Karl-Erich
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700 1 _ |0 P:(DE-Juel1)131426
|a Drepper, Thomas
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773 _ _ |0 PERI:(DE-600)2480063-6
|a 10.1039/c4ib00027g
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|t Integrative biology
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|y 2014
856 4 _ |u https://juser.fz-juelich.de/record/153998/files/FZJ-2014-03427.pdf
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