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@ARTICLE{Delatte:15400,
author = {Delatte, T.L. and Sedijani, P. and Kondou, Y. and Matsui,
M. and de Jong, G.J. and Somsen, G.W. and Wiese-Klinkenberg,
A. and Primavesi, L.F. and Paul, M.J. and Schluepmann, H.},
title = {{G}rowth arrest by trehalose-6-phosphate: an astonishing
case of primary metabolite control over growth by way of the
{S}n{RK}1 signaling pathway},
journal = {Plant physiology},
volume = {157},
issn = {0032-0889},
address = {Rockville, Md.: Soc.},
publisher = {JSTOR},
reportid = {PreJuSER-15400},
pages = {160 - 174},
year = {2011},
note = {This work was supported by Chemische Wetenschappen-ECHO (to
T.L.D.), Netherlands Organisation for International
Cooperation in Higher Education-PhD (to P.S.), the
Biotechnological and Biological Sciences Research Council of
the United Kingdom (grant no. BB/D006112/1 to L.F.P. and
M.J.P.), Netherlands Organisation for Scientific Research
Meervoud, and Utrecht University Cooperation Start-Up Fund
Asia (to H.S.).},
abstract = {The strong regulation of plant carbon allocation and growth
by trehalose metabolism is important for our understanding
of the mechanisms that determine growth and yield, with
obvious applications in crop improvement. To gain further
insight on the growth arrest by trehalose feeding, we first
established that starch-deficient seedlings of the plastidic
phosphoglucomutase1 mutant were similarly affected as the
wild type on trehalose. Starch accumulation in the source
cotyledons, therefore, did not cause starvation and
consequent growth arrest in the growing zones. We then
screened the FOX collection of Arabidopsis (Arabidopsis
thaliana) expressing full-length cDNAs for seedling
resistance to 100 mm trehalose. Three independent transgenic
lines were identified with dominant segregation of the
trehalose resistance trait that overexpress the bZIP11 (for
basic region/leucine zipper motif) transcription factor. The
resistance of these lines to trehalose could not be
explained simply through enhanced trehalase activity or
through inhibition of bZIP11 translation. Instead,
trehalose-6-phosphate (T6P) accumulation was much increased
in bZIP11-overexpressing lines, suggesting that these lines
may be insensitive to the effects of T6P. T6P is known to
inhibit the central stress-integrating kinase SnRK1 (KIN10)
activity. We confirmed that this holds true in extracts from
seedlings grown on trehalose, then showed that two
independent transgenic lines overexpressing KIN10 were
insensitive to trehalose. Moreover, the expression of marker
genes known to be jointly controlled by SnRK1 activity and
bZIP11 was consistent with low SnRK1 or bZIP11 activity in
seedlings on trehalose. These results reveal an astonishing
case of primary metabolite control over growth by way of the
SnRK1 signaling pathway involving T6P, SnRK1, and bZIP11.},
keywords = {Arabidopsis: genetics / Arabidopsis: metabolism /
Arabidopsis Proteins: genetics / Arabidopsis Proteins:
metabolism / Basic-Leucine Zipper Transcription Factors:
genetics / DNA, Complementary / Plants, Genetically Modified
/ Protein Biosynthesis / Protein-Serine-Threonine Kinases:
metabolism / Signal Transduction: drug effects / Sugar
Phosphates: metabolism / Trehalose: analogs $\&$ derivatives
/ Trehalose: metabolism / ATB2 protein, Arabidopsis (NLM
Chemicals) / Arabidopsis Proteins (NLM Chemicals) /
Basic-Leucine Zipper Transcription Factors (NLM Chemicals) /
DNA, Complementary (NLM Chemicals) / Sugar Phosphates (NLM
Chemicals) / trehalose-6-phosphate (NLM Chemicals) /
Trehalose (NLM Chemicals) / Protein-Serine-Threonine Kinases
(NLM Chemicals) / SnRK1 protein, Arabidopsis (NLM Chemicals)
/ J (WoSType)},
cin = {IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21753116},
pmc = {pmc:PMC3165867},
UT = {WOS:000294491800013},
doi = {10.1104/pp.111.180422},
url = {https://juser.fz-juelich.de/record/15400},
}