Journal Article

PreJuSER-15400

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway

Delatte, T. L. ; Sedijani, P. ; Kondou, Y. ; Matsui, M. ; de Jong, G. J. ; Somsen, G. W. ; Wiese-Klinkenberg, A.FZJ* ; Primavesi, L. F. ; Paul, M. J. ; Schluepmann, H.

2011
JSTOR Rockville, Md.: Soc.

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Please use a persistent id in citations: doi:10.1104/pp.111.180422

Abstract: The strong regulation of plant carbon allocation and growth by trehalose metabolism is important for our understanding of the mechanisms that determine growth and yield, with obvious applications in crop improvement. To gain further insight on the growth arrest by trehalose feeding, we first established that starch-deficient seedlings of the plastidic phosphoglucomutase1 mutant were similarly affected as the wild type on trehalose. Starch accumulation in the source cotyledons, therefore, did not cause starvation and consequent growth arrest in the growing zones. We then screened the FOX collection of Arabidopsis (Arabidopsis thaliana) expressing full-length cDNAs for seedling resistance to 100 mm trehalose. Three independent transgenic lines were identified with dominant segregation of the trehalose resistance trait that overexpress the bZIP11 (for basic region/leucine zipper motif) transcription factor. The resistance of these lines to trehalose could not be explained simply through enhanced trehalase activity or through inhibition of bZIP11 translation. Instead, trehalose-6-phosphate (T6P) accumulation was much increased in bZIP11-overexpressing lines, suggesting that these lines may be insensitive to the effects of T6P. T6P is known to inhibit the central stress-integrating kinase SnRK1 (KIN10) activity. We confirmed that this holds true in extracts from seedlings grown on trehalose, then showed that two independent transgenic lines overexpressing KIN10 were insensitive to trehalose. Moreover, the expression of marker genes known to be jointly controlled by SnRK1 activity and bZIP11 was consistent with low SnRK1 or bZIP11 activity in seedlings on trehalose. These results reveal an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway involving T6P, SnRK1, and bZIP11.

Keyword(s): Arabidopsis: genetics (MeSH) ; Arabidopsis: metabolism (MeSH) ; Arabidopsis Proteins: genetics (MeSH) ; Arabidopsis Proteins: metabolism (MeSH) ; Basic-Leucine Zipper Transcription Factors: genetics (MeSH) ; DNA, Complementary (MeSH) ; Plants, Genetically Modified (MeSH) ; Protein Biosynthesis (MeSH) ; Protein-Serine-Threonine Kinases: metabolism (MeSH) ; Signal Transduction: drug effects (MeSH) ; Sugar Phosphates: metabolism (MeSH) ; Trehalose: analogs & derivatives (MeSH) ; Trehalose: metabolism (MeSH) ; ATB2 protein, Arabidopsis ; Arabidopsis Proteins ; Basic-Leucine Zipper Transcription Factors ; DNA, Complementary ; Sugar Phosphates ; trehalose-6-phosphate ; Trehalose ; Protein-Serine-Threonine Kinases ; SnRK1 protein, Arabidopsis ; J

Note: This work was supported by Chemische Wetenschappen-ECHO (to T.L.D.), Netherlands Organisation for International Cooperation in Higher Education-PhD (to P.S.), the Biotechnological and Biological Sciences Research Council of the United Kingdom (grant no. BB/D006112/1 to L.F.P. and M.J.P.), Netherlands Organisation for Scientific Research Meervoud, and Utrecht University Cooperation Start-Up Fund Asia (to H.S.).

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Contributing Institute(s):

1. Pflanzenwissenschaften (IBG-2)

Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2011

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