Journal Article

FZJ-2014-03748

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Expression profile of apoptosis related genes during differentiation in the human neuronal progenitor cell line VM197

Jaeger, A. (Corresponding Author)FZJ* ; Klum, S. ; Weiss, D. G. ; Kriehuber, R.FZJ*

2008
Elsevier München

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Please use a persistent id in citations: doi:10.1016/j.ejcb.2008.01.004

Abstract: The development of the human central nervous system is accompanied by cell proliferation, differentiation and cell death. The massive loss of neural cells during neurogenesis suggests that apoptosis plays a critical role in human brain development. To characterize apoptotic processes during differentiation we analyzed the gene expression profiles of proliferating versus differentiating ReNCell VM197 at six different time points after induction of differentiation in vitro. The expression levels of about 1,117 cDNA/ESTs (expressed sequence tags) related to apoptosis pathways were found to be changed at least 2-fold higher or lower in differentiating cells than in proliferating cells. Consistent with our previous Western/Immunoblotting studies, we observed that pro-apoptotic proteins like Bax, Bid and cytochrome c showed increased gene expression during the first 6 h of differentiation and diminished expression levels at later stages of differentiation. In contrast the gene and protein expression levels of the anti-apoptotic protein Bcl-2 was found to be increased 6 h and 12 h respectively post-initiation of differentiation. We conclude that both, pro-apoptotic and anti-apoptotic processes occur during differentiation of VM197 cells and that Bcl-2 plays a protective role against apoptosis in fully differentiated neurons.

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Contributing Institute(s):

1. Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie (S-US)

Research Program(s):

1. 899 - ohne Topic (POF2-899) (POF2-899)

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; BIOSIS Previews ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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