TY  - JOUR
AU  - Kriehuber, Ralf
AU  - von Ameln, Marcel
AU  - Bahn, S.
AU  - Pomplun, Ekkehard
TI  - Increased Micronucleus Induction in 170Tm-irradiated Nanogold-labeled SCL II-cells
JO  - Radioprotection
VL  - 43
IS  - 5
SN  - 1769-700X
CY  - Les Ulis
PB  - EDP Sciences
M1  - FZJ-2014-03877
SP  - 271
PY  - 2008
AB  - Photoelectric absorption of photons with energies slightly above the K shell binding energy of an appropriate element can result in the emission of a shower of lowenergy Auger-electrons. It is well known that those electrons released by Augerelectron-emitting radionuclides located in the immediate vicinity of the DNA cause high-LET-type damage and induce an enhanced relative biological effectiveness when compared to low-LET radiation. Therefore, an enhanced biological effectiveness is expected after photon activation as well. To proof if photoelectric absorptionleads to an increased cellular radiotoxicity we investigated in SCL II-cells whether photon activation of intracellular located nano-sized gold particles is feasibleto enhance cyto- and genotoxic effects in vitro. SCL II-cells were transfected with colloidal nano-sized gold particles (40 nm) and gold-labeled DNA-triplexforming-oligonucleotides (TFO) and irradiated with a suitable 170Tm source (micro seeds). Genotoxicity was assessed using the Micronucleus-Assay and cytotoxicity was investigated using the Colony-Forming-Assay. Preliminary results indicate that Nanogold-labeled SCL II-cells show a 2-fold increase in micronucleus formation when compared to irradiated non-labeled cells. Non-irradiated NanogoldlabeledSCL II-cells showed the same background level of micronucleated cells as non-labeled SCLII-cells. The mitotic activity was neither disturbed by the goldlabeling nor the transfection procedure. Cytotoxic effects are less prominent but still need further investigation. Photon activation might be a promising approach to increase the biological effectiveness of low-LET-radiation and might be of great value for new brachytherapy strategies.This work is financially supported by STEP Pockau GmbH
T2  - 36th Annual Meeting of the European Radiation Research Society
CY  - ,  ()
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1051/radiopro:2008681
UR  - https://juser.fz-juelich.de/record/154583
ER  -