Journal Article

FZJ-2014-03877

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Increased Micronucleus Induction in 170Tm-irradiated Nanogold-labeled SCL II-cells

Kriehuber, R. (Corresponding author)FZJ* ; von Ameln, M.FZJ* ; Bahn, S. ; Pomplun, E.

2008
EDP Sciences Les Ulis

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Please use a persistent id in citations: http://hdl.handle.net/2128/16293  doi:10.1051/radiopro:2008681

Abstract: Photoelectric absorption of photons with energies slightly above the K shell binding energy of an appropriate element can result in the emission of a shower of lowenergy Auger-electrons. It is well known that those electrons released by Augerelectron-emitting radionuclides located in the immediate vicinity of the DNA cause high-LET-type damage and induce an enhanced relative biological effectiveness when compared to low-LET radiation. Therefore, an enhanced biological effectiveness is expected after photon activation as well. To proof if photoelectric absorptionleads to an increased cellular radiotoxicity we investigated in SCL II-cells whether photon activation of intracellular located nano-sized gold particles is feasibleto enhance cyto- and genotoxic effects in vitro. SCL II-cells were transfected with colloidal nano-sized gold particles (40 nm) and gold-labeled DNA-triplexforming-oligonucleotides (TFO) and irradiated with a suitable 170Tm source (micro seeds). Genotoxicity was assessed using the Micronucleus-Assay and cytotoxicity was investigated using the Colony-Forming-Assay. Preliminary results indicate that Nanogold-labeled SCL II-cells show a 2-fold increase in micronucleus formation when compared to irradiated non-labeled cells. Non-irradiated NanogoldlabeledSCL II-cells showed the same background level of micronucleated cells as non-labeled SCLII-cells. The mitotic activity was neither disturbed by the goldlabeling nor the transfection procedure. Cytotoxic effects are less prominent but still need further investigation. Photon activation might be a promising approach to increase the biological effectiveness of low-LET-radiation and might be of great value for new brachytherapy strategies.This work is financially supported by STEP Pockau GmbH

Keyword(s): Biology (2nd)

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Contributing Institute(s):

1. Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie (S-US)

Research Program(s):

1. 899 - ohne Topic (POF2-899) (POF2-899)

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Database coverage:
; ; JCR ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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