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@ARTICLE{Kriehuber:154583,
      author       = {Kriehuber, Ralf and von Ameln, Marcel and Bahn, S. and
                      Pomplun, Ekkehard},
      title        = {{I}ncreased {M}icronucleus {I}nduction in
                      170{T}m-irradiated {N}anogold-labeled {SCL} {II}-cells},
      journal      = {Radioprotection},
      volume       = {43},
      number       = {5},
      issn         = {1769-700X},
      address      = {Les Ulis},
      publisher    = {EDP Sciences},
      reportid     = {FZJ-2014-03877},
      pages        = {271},
      year         = {2008},
      abstract     = {Photoelectric absorption of photons with energies slightly
                      above the K shell binding energy of an appropriate element
                      can result in the emission of a shower of lowenergy
                      Auger-electrons. It is well known that those electrons
                      released by Augerelectron-emitting radionuclides located in
                      the immediate vicinity of the DNA cause high-LET-type damage
                      and induce an enhanced relative biological effectiveness
                      when compared to low-LET radiation. Therefore, an enhanced
                      biological effectiveness is expected after photon activation
                      as well. To proof if photoelectric absorptionleads to an
                      increased cellular radiotoxicity we investigated in SCL
                      II-cells whether photon activation of intracellular located
                      nano-sized gold particles is feasibleto enhance cyto- and
                      genotoxic effects in vitro. SCL II-cells were transfected
                      with colloidal nano-sized gold particles (40 nm) and
                      gold-labeled DNA-triplexforming-oligonucleotides (TFO) and
                      irradiated with a suitable 170Tm source (micro seeds).
                      Genotoxicity was assessed using the Micronucleus-Assay and
                      cytotoxicity was investigated using the
                      Colony-Forming-Assay. Preliminary results indicate that
                      Nanogold-labeled SCL II-cells show a 2-fold increase in
                      micronucleus formation when compared to irradiated
                      non-labeled cells. Non-irradiated NanogoldlabeledSCL
                      II-cells showed the same background level of micronucleated
                      cells as non-labeled SCLII-cells. The mitotic activity was
                      neither disturbed by the goldlabeling nor the transfection
                      procedure. Cytotoxic effects are less prominent but still
                      need further investigation. Photon activation might be a
                      promising approach to increase the biological effectiveness
                      of low-LET-radiation and might be of great value for new
                      brachytherapy strategies.This work is financially supported
                      by STEP Pockau GmbH},
      organization  = {36th Annual Meeting of the European
                       Radiation Research Society,},
      cin          = {S-US},
      ddc          = {530},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)16},
      doi          = {10.1051/radiopro:2008681},
      url          = {https://juser.fz-juelich.de/record/154583},
}