Comparison of Individual Radiosensitivity of PBL from Prostate Cancer Patients and Healthy Donors

Brzozowska, K.; Schmitz, Sabine; Wojcik, A.; Kriehuber, R.

doi:10.1051/radiopro:2008664

Journal Article

FZJ-2014-03878

Comparison of Individual Radiosensitivity of PBL from Prostate Cancer Patients and Healthy Donors

Brzozowska, K. (Corresponding Author) ; Wojcik, A. ; Kriehuber, R.FZJ* ; Schmitz, S.FZJ*

2008
EDP Sciences Les Ulis

36th Annual Meeting of the European Radiation Research Society, ERRS, Tours, France, Radioprotection 43(5), p.241 (2008) [10.1051/radiopro:2008664]

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Abstract: Individual radiosensitivity is a biological feature distributed heterogeneously withinthe population. Approximately 10 % of people have an enhanced intrinsic radiosensitvityand hence a higher risk for developing side effects during radiotherapy. Theunderlying mechanisms remain unclear. DNA repair deficiency and altered apoptosischaracteristics are discussed to be markers for radiosensitivity that can easilybe analysed in peripheral blood lymphocytes (PBL). The aim of our study is tofind out whether PBL from cancer patients with strong clinical side effects underradiotherapy, as assessed clinically on the basis of the RTOG/EORTC scale,show enhanced rates of double strand breaks (dsb), decreased DNA repair capacityand altered induction of apoptosis in vitro when compared to lymphocytes frompatients without side effects and age-matched healthy donors. Additionally, toinvestigate whether the in vitro radiosensitivity of PBL is a marker of prostatecancer predisposition the data of patients are analysed versus the data of PBLcollected from healthy donors. To achieve this goal blood samples are collected,exposed to a dose of 1 Gy or 0.5 Gy and the following biological endpoints are analysed:the initial level of dsb and the repair kinetics (°-H2AX-Assay), apoptosis(Annexin V-Assay) and the induction of chromatid-type chromosomal aberrations(G2-Assay). Preliminary results reveal that the maximum induction of foci asmeasured by °-H2AX-Assay occurs 30 min after irradiation. 24 h after irradiationthe amount of foci declines to approximately control level. FACS data confirmthese findings. First results derived from the Annexin V-Assay show a significantincrease of early apoptosis 24 h after irradiation compared to the controls.

Keyword(s): Biology (2nd)

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