TY  - JOUR
AU  - Viale-Bouroncle, S.
AU  - Völlner, F.
AU  - Möhl, C.
AU  - Küpper, K.
AU  - Brockhoff, G.
AU  - Reichert, T.E.
AU  - Schmalz, G.
AU  - Morsczeck, C.
TI  - Soft matrix supports osteogenic differentiation of human dental follicle cells
JO  - Biochemical and biophysical research communications
VL  - 410
SN  - 0006-291X
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-15654
SP  - 587 - 592
PY  - 2011
N1  - Record converted from VDB: 12.11.2012
AB  - The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.
KW  - Cell Culture Techniques
KW  - Cell Differentiation
KW  - Cell Proliferation
KW  - Cells, Cultured
KW  - Culture Media: pharmacology
KW  - Dental Sac: cytology
KW  - Dental Sac: drug effects
KW  - Dexamethasone: pharmacology
KW  - Humans
KW  - Male
KW  - Mesenchymal Stem Cells: cytology
KW  - Mesenchymal Stem Cells: drug effects
KW  - Osteogenesis
KW  - Surface Properties
KW  - Young Adult
KW  - Culture Media (NLM Chemicals)
KW  - Dexamethasone (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21684253
UR  - <Go to ISI:>//WOS:000292797700037
DO  - DOI:10.1016/j.bbrc.2011.06.031
UR  - https://juser.fz-juelich.de/record/15654
ER  -