Journal Article

PreJuSER-15654

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Soft matrix supports osteogenic differentiation of human dental follicle cells

Viale-Bouroncle, S. ; Völlner, F. ; Möhl, C. ; Küpper, K.FZJ* ; Brockhoff, G. ; Reichert, T. E.FZJ* ; Schmalz, G.FZJ* ; Morsczeck, C.FZJ*

2011
Academic Press Orlando, Fla.

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Please use a persistent id in citations: doi:10.1016/j.bbrc.2011.06.031

Abstract: The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs.

Keyword(s): Cell Culture Techniques (MeSH) ; Cell Differentiation (MeSH) ; Cell Proliferation (MeSH) ; Cells, Cultured (MeSH) ; Culture Media: pharmacology (MeSH) ; Dental Sac: cytology (MeSH) ; Dental Sac: drug effects (MeSH) ; Dexamethasone: pharmacology (MeSH) ; Humans (MeSH) ; Male (MeSH) ; Mesenchymal Stem Cells: cytology (MeSH) ; Mesenchymal Stem Cells: drug effects (MeSH) ; Osteogenesis (MeSH) ; Surface Properties (MeSH) ; Young Adult (MeSH) ; Culture Media ; Dexamethasone ; J ; Dental follicle cells (auto) ; PDMS (auto) ; Differentiation (auto) ; Proliferation (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biomechanik (ICS-7)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2011

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