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@ARTICLE{VialeBouroncle:15654,
author = {Viale-Bouroncle, S. and Völlner, F. and Möhl, C. and
Küpper, K. and Brockhoff, G. and Reichert, T.E. and
Schmalz, G. and Morsczeck, C.},
title = {{S}oft matrix supports osteogenic differentiation of human
dental follicle cells},
journal = {Biochemical and biophysical research communications},
volume = {410},
issn = {0006-291X},
address = {Orlando, Fla.},
publisher = {Academic Press},
reportid = {PreJuSER-15654},
pages = {587 - 592},
year = {2011},
note = {Record converted from VDB: 12.11.2012},
abstract = {The differentiation of stem cells can be directed by the
grade of stiffness of the developed tissue cells. For
example a rigid extracellular matrix supports the osteogenic
differentiation in bone marrow derived mesenchymal stem
cells (MSCs). However, less is known about the relation of
extracellular matrix stiffness and cell differentiation of
ectomesenchymal dental precursor cells. Our study examined
for the first time the influence of the surface stiffness on
the proliferation and osteogenic differentiation of human
dental follicle cells (DFCs). Cell proliferation of DFCs was
only slightly decreased on cell culture surfaces with a
bone-like stiffness. The osteogenic differentiation in DFCs
could only be initiated with a dexamethasone based
differentiation medium after using varying stiffness. Here,
the softest surface improved the induction of osteogenic
differentiation in comparison to that with the highest
stiffness. In conclusion, different to bone marrow derived
MSCs, soft ECMs have a superior capacity to support the
osteogenic differentiation of DFCs.},
keywords = {Cell Culture Techniques / Cell Differentiation / Cell
Proliferation / Cells, Cultured / Culture Media:
pharmacology / Dental Sac: cytology / Dental Sac: drug
effects / Dexamethasone: pharmacology / Humans / Male /
Mesenchymal Stem Cells: cytology / Mesenchymal Stem Cells:
drug effects / Osteogenesis / Surface Properties / Young
Adult / Culture Media (NLM Chemicals) / Dexamethasone (NLM
Chemicals) / J (WoSType)},
cin = {ICS-7},
ddc = {570},
cid = {I:(DE-Juel1)ICS-7-20110106},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK505},
shelfmark = {Biochemistry $\&$ Molecular Biology / Biophysics},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21684253},
UT = {WOS:000292797700037},
doi = {10.1016/j.bbrc.2011.06.031},
url = {https://juser.fz-juelich.de/record/15654},
}