TY  - JOUR
AU  - Kramer, B.
AU  - Schulte, D.
AU  - Korner, C.
AU  - Zwank, C.
AU  - Hartmann, A.
AU  - Michalk, M.
AU  - Sohne, J.
AU  - Langhans, B.
AU  - Nischalke, H.D.
AU  - Coenen, M.
AU  - Möhl, C.
AU  - Vogt, A.
AU  - Hennenberg, M.
AU  - Sauerbruch, T.
AU  - Spengler, U.
AU  - Nattermann, J.
TI  - Regulation of NK Cell Trafficking by CD81
JO  - European journal of immunology
VL  - 39
SN  - 0014-2980
CY  - Weinheim
PB  - Wiley-VCH
M1  - PreJuSER-15797
SP  - 3447 - 3458
PY  - 2009
N1  - This work was supported by a grant from BMBF (German Ministry for Science and Education) (01KI0791). We thank Dirk Stabenow from the Institute of Molecular Medicine and Experimental Immunology, University of Bonn, for his support performing calcium influx.
AB  - NK cells, a heterogeneous sub-population of lymphocytes, are critically involved in the regulation of both innate and adaptive immune responses in humans. Besides their participation in the control of tumors and viral infections, they also regulate inflammatory processes, mediating both beneficial and detrimental effects. To effectively fulfil their role in immune surveillance, proper trafficking of NK cells is essential. However, the mechanisms and factors governing NK cell recruitment are only poorly dissected. Here, we describe the functional role of tetraspanins, a family of evolutionary conserved cell-surface proteins, in modulating migration and transmigration of human NK cells. We demonstrate expression of various tetraspanins on NK cells. Furthermore, we show that stimulation of the NK cell-expressed tetraspanin CD81 induces phosphorylation of ezrin/radixin/moesin proteins and leads to NK cell polarization thereby facilitating NK cell migration toward various chemokines/cytokines. Finally, we provide evidence for a role of CD81 in promoting adhesion of NK cells to components of the extracellular matrix, a prerequisite for extravasation of lymphocytes in inflamed tissues. Thus, our data suggest that the tetraspanin CD81 is importantly involved in the regulation of NK cell recruitment.
KW  - Antigens, CD: metabolism
KW  - Antigens, CD63
KW  - Antigens, CD81
KW  - Blotting, Western
KW  - Cell Adhesion
KW  - Cell Movement
KW  - Cell Polarity
KW  - Chemokine CCL5: metabolism
KW  - Chemokine CCL5: pharmacology
KW  - Chemotaxis: drug effects
KW  - Cytoskeletal Proteins: metabolism
KW  - Flow Cytometry
KW  - Humans
KW  - Killer Cells, Natural: cytology
KW  - Killer Cells, Natural: metabolism
KW  - Membrane Proteins: metabolism
KW  - Microfilament Proteins: metabolism
KW  - Phosphorylation
KW  - Platelet Membrane Glycoproteins: metabolism
KW  - Protein Binding
KW  - Protein Kinase C: metabolism
KW  - rho-Associated Kinases: metabolism
KW  - Antigens, CD (NLM Chemicals)
KW  - Antigens, CD63 (NLM Chemicals)
KW  - Antigens, CD81 (NLM Chemicals)
KW  - CD63 protein, human (NLM Chemicals)
KW  - CD81 protein, human (NLM Chemicals)
KW  - Chemokine CCL5 (NLM Chemicals)
KW  - Cytoskeletal Proteins (NLM Chemicals)
KW  - Membrane Proteins (NLM Chemicals)
KW  - Microfilament Proteins (NLM Chemicals)
KW  - Platelet Membrane Glycoproteins (NLM Chemicals)
KW  - ezrin (NLM Chemicals)
KW  - moesin (NLM Chemicals)
KW  - radixin (NLM Chemicals)
KW  - rho-Associated Kinases (NLM Chemicals)
KW  - Protein Kinase C (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19830727
UR  - <Go to ISI:>//WOS:000272928300021
DO  - DOI:10.1002/eji.200939234
UR  - https://juser.fz-juelich.de/record/15797
ER  -