Journal Article

PreJuSER-15797

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Regulation of NK Cell Trafficking by CD81

Kramer, B. ; Schulte, D. ; Korner, C. ; Zwank, C. ; Hartmann, A. ; Michalk, M. ; Sohne, J. ; Langhans, B. ; Nischalke, H. D. ; Coenen, M. ; Möhl, C.FZJ* ; Vogt, A. ; Hennenberg, M. ; Sauerbruch, T. ; Spengler, U. ; Nattermann, J.

2009
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/eji.200939234

Abstract: NK cells, a heterogeneous sub-population of lymphocytes, are critically involved in the regulation of both innate and adaptive immune responses in humans. Besides their participation in the control of tumors and viral infections, they also regulate inflammatory processes, mediating both beneficial and detrimental effects. To effectively fulfil their role in immune surveillance, proper trafficking of NK cells is essential. However, the mechanisms and factors governing NK cell recruitment are only poorly dissected. Here, we describe the functional role of tetraspanins, a family of evolutionary conserved cell-surface proteins, in modulating migration and transmigration of human NK cells. We demonstrate expression of various tetraspanins on NK cells. Furthermore, we show that stimulation of the NK cell-expressed tetraspanin CD81 induces phosphorylation of ezrin/radixin/moesin proteins and leads to NK cell polarization thereby facilitating NK cell migration toward various chemokines/cytokines. Finally, we provide evidence for a role of CD81 in promoting adhesion of NK cells to components of the extracellular matrix, a prerequisite for extravasation of lymphocytes in inflamed tissues. Thus, our data suggest that the tetraspanin CD81 is importantly involved in the regulation of NK cell recruitment.

Keyword(s): Antigens, CD: metabolism (MeSH) ; Antigens, CD63 (MeSH) ; Antigens, CD81 (MeSH) ; Blotting, Western (MeSH) ; Cell Adhesion (MeSH) ; Cell Movement (MeSH) ; Cell Polarity (MeSH) ; Chemokine CCL5: metabolism (MeSH) ; Chemokine CCL5: pharmacology (MeSH) ; Chemotaxis: drug effects (MeSH) ; Cytoskeletal Proteins: metabolism (MeSH) ; Flow Cytometry (MeSH) ; Humans (MeSH) ; Killer Cells, Natural: cytology (MeSH) ; Killer Cells, Natural: metabolism (MeSH) ; Membrane Proteins: metabolism (MeSH) ; Microfilament Proteins: metabolism (MeSH) ; Phosphorylation (MeSH) ; Platelet Membrane Glycoproteins: metabolism (MeSH) ; Protein Binding (MeSH) ; Protein Kinase C: metabolism (MeSH) ; rho-Associated Kinases: metabolism (MeSH) ; Antigens, CD ; Antigens, CD63 ; Antigens, CD81 ; CD63 protein, human ; CD81 protein, human ; Chemokine CCL5 ; Cytoskeletal Proteins ; Membrane Proteins ; Microfilament Proteins ; Platelet Membrane Glycoproteins ; ezrin ; moesin ; radixin ; rho-Associated Kinases ; Protein Kinase C ; J ; Adhesion (auto) ; Cell migration (auto) ; Cell trafficking (auto) ; Chemokines (auto) ; NK cells (auto)

Note: This work was supported by a grant from BMBF (German Ministry for Science and Education) (01KI0791). We thank Dirk Stabenow from the Institute of Molecular Medicine and Experimental Immunology, University of Bonn, for his support performing calcium influx.

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Contributing Institute(s):

1. Biomechanik (ICS-7)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2009
Notes: Nachtrag

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Database coverage:
; JCR ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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