Gast ::
| Hauptseite > Publikationsdatenbank > Element imaging in formalin fixed slices of human mesencephalon |
| Hauptseite > Publikationsdatenbank > Element imaging in formalin fixed slices of human mesencephalon |
| Journal Article | PreJuSER-16497 |
; ;
2011
Elsevier Science
[S.l.]
This record in other databases: 
Please use a persistent id in citations: doi:10.1016/j.ijms.2011.03.006
Abstract: The scarcity of native frozen pathological and healthy human brain tissue is actually one of the major bottlenecks concerning the investigation of neuropsychiatric diseases. Therefore it is of some interest to make formalin fixed tissue specimens accessible to mass spectrometric analytical techniques. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and matrix matched standards were employed to generate quantitative element maps of human mesencephalon slices that had been stored in formalin for more than 10 years of a single subject. The characteristic distribution patterns of Cu and Zn were completely blunted but Fe was clearly enriched in the substantia nigra (SN) - especially in the matrix of the pars compacta - and the nucleus ruber (NR) at concentrations of 179 mu g g(-1), 231 mu g g(-1) and 187 mu g g(-1) (wet weight), respectively, very similar to literature values obtained from homogenates of fresh tissue. Mn and Ca congruently reflected the distribution of melanised SN neurons. Interestingly, the distribution of Pb was congruent to that of Fe reaching 0.8 mu g g(-1) in the SN, 1.1 mu g g(-1) in the matrix and 0.9 mu g g(-1) in the NR. Images of C and P reflected the gray matter-white matter partition of the tissue. These results suggest that a meaningful quantitative assessment of Fe, Mn and Pb is possible in tissue samples which have previously been stored in buffered formalin solution. (C) 2011 Elsevier B.V. All rights reserved.
Keyword(s): J ; Human brain tissue (auto) ; LA-ICP-MS bioimaging (auto) ; Metal imaging (auto) ; Quantitative distribution analysis (auto) ; Substantia nigra (auto)
|
The record appears in these collections: |