%0 Journal Article
%A Hucklenbroich, J.
%A Klein, R.
%A Neumaier, B.
%A Graf, R.
%A Fink, G. R.
%A Schroeter, M.
%A Rueger, M. A.
%T Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
%J Stem cell research & therapy
%V 5
%N 4
%@ 1757-6512
%C London
%I BioMed Central
%M FZJ-2014-05381
%P Article 100 (1-9)
%D 2014
%X IntroductionAromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated.MethodsWe exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [18F]-fluoro-L-thymidine ([18F]FLT), as well as ex vivo.ResultsIn vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [18F]FLT-PET and histology (P < 0.05).ConclusionsBoth in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000344567300001
%$ pmid:25928248
%R 10.1186/scrt500
%U https://juser.fz-juelich.de/record/171822