Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.

Hucklenbroich, J.; Graf, R.; Rueger, M. A.; Schroeter, M.; Neumaier, B.; Fink, G. R.; Klein, R.

doi:10.1186/scrt500

Journal Article

FZJ-2014-05381

Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.

Hucklenbroich, J. (Corresponding Author) ; Klein, R. ; Neumaier, B. ; Graf, R. ; Fink, G. R.FZJ* ; Schroeter, M. ; Rueger, M. A.

2014
BioMed Central London

Stem cell research & therapy 5(4), Article 100 (1-9) (2014) [10.1186/scrt500]

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Please use a persistent id in citations: http://hdl.handle.net/2128/8034 doi:10.1186/scrt500

Abstract: IntroductionAromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated.MethodsWe exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [18F]-fluoro-L-thymidine ([18F]FLT), as well as ex vivo.ResultsIn vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [18F]FLT-PET and histology (P < 0.05).ConclusionsBoth in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.

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