TY  - JOUR
AU  - Hucklenbroich, J.
AU  - Klein, R.
AU  - Neumaier, B.
AU  - Graf, R.
AU  - Fink, G. R.
AU  - Schroeter, M.
AU  - Rueger, M. A.
TI  - Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.
JO  - Stem cell research & therapy
VL  - 5
IS  - 4
SN  - 1757-6512
CY  - London
PB  - BioMed Central
M1  - FZJ-2014-05381
SP  - Article 100 (1-9)
PY  - 2014
AB  - IntroductionAromatic (ar-) turmerone is a major bioactive compound of the herb Curcuma longa. It has been suggested that ar-turmerone inhibits microglia activation, a property that may be useful in treating neurodegenerative disease. Furthermore, the effects of ar-turmerone on neural stem cells (NSCs) remain to be investigated.MethodsWe exposed primary fetal rat NSCs to various concentrations of ar-turmerone. Thereafter, cell proliferation and differentiation potential were assessed. In vivo, naïve rats were treated with a single intracerebroventricular (i.c.v.) injection of ar-turmerone. Proliferative activity of endogenous NSCs was assessed in vivo, by using noninvasive positron emission tomography (PET) imaging and the tracer [18F]-fluoro-L-thymidine ([18F]FLT), as well as ex vivo.ResultsIn vitro, ar-turmerone increased dose-dependently the number of cultured NSCs, because of an increase in NSC proliferation (P < 0.01). Proliferation data were supported by qPCR-data for Ki-67 mRNA. In vitro as well as in vivo, ar-turmerone promoted neuronal differentiation of NSCs. In vivo, after i.c.v. injection of ar-turmerone, proliferating NSCs were mobilized from the subventricular zone (SVZ) and the hippocampus of adult rats, as demonstrated by both [18F]FLT-PET and histology (P < 0.05).ConclusionsBoth in vitro and in vivo data suggest that ar-turmerone induces NSC proliferation. Ar-turmerone thus constitutes a promising candidate to support regeneration in neurologic disease.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000344567300001
C6  - pmid:25928248
DO  - DOI:10.1186/scrt500
UR  - https://juser.fz-juelich.de/record/171822
ER  -